Plant regeneration in weeping lovegrass, (Eragrostis curvula) through inflorescence culture

被引：16

作者：

Echenique, V ^{[1
]}

Polci, P ^{[1
]}

Mroginski, L ^{[1
]}

机构：

[1] UNIV NACL NORDESTE,INST BOT,FAC CS AGR,RA-3400 CORRIENTES,ARGENTINA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1996年 / 46卷 / 02期

关键词：

apomixis; grasses; organogenesis; somatic embryogenesis;

D O I：

10.1007/BF00034845

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant regeneration from four genotypes of weeping lovegrass (Eragrostis curvula (Schrad.) Nees), is reported via three developmental pathways: embryogenesis, organogenesis and direct regeneration. Organogenic and embryogenic callus cultures were initiated from young inflorescence segments on Murashige and Skoog's medium supplemented with 2,4-D and BA at different concentrations. The most suitable concentrations of 2,4-D for callus growth and development were 9 and Is mu M combined with a BA concentration of 0.044 mu M. Genotypical differences were observed in the morphogenetic capacity. Direct regeneration was observed under similar culture conditions (culture medium, temperature and photoperiod) but with high light intensity (66 mu mol m(-2) s(-1)). Young plants were successfully transplanted to pots and grown to maturity in the greenhouse.

引用

页码：123 / 130

页数：8

共 27 条

[1] EMBRYOGENESIS FROM CULTURED IMMATURE INFLORESCENCES OF SORGHUM-BICOLOR [J].

BRETTELL, RIS ;

WERNICKE, W ;

THOMAS, E .

PROTOPLASMA, 1980, 104 (1-2) :141-148

[2]

Covas G., 1991, PASTO LLORON SU BIOL, P7

[3]

DALE P J, 1981, Plant Cell Tissue and Organ Culture, V1, P47, DOI 10.1007/BF02318903

[4] IMMATURE INFLORESCENCE CULTURE IN LOLIUM, FESTUCA, PHLEUM AND DACTYLIS [J].

DALE, PJ ;

DALTON, SJ .

ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1983, 111 (01) :39-45

[5] STUDY OF CALLUS GROWTH AND ORGAN FORMATION IN WHEAT (TRITICUM-AESTIVUM) TISSUE-CULTURES [J].

DUDITS, D ;

NEMET, G ;

HAYDU, Z .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1975, 53 (10) :957-963

[6]

ECHENIQUE V, 1987, THESIS U NACL SUR

[7] BIOCHEMICAL MARKERS OF EMBRYOGENESIS IN TISSUE-CULTURES OF THE MAIZE INBRED B73 [J].

EVERETT, NP ;

WACH, MJ ;

ASHWORTH, DJ .

PLANT SCIENCE, 1985, 41 (02) :133-140

[8]

GEIER T, 1991, GENETICS BREEDING OR, P179

[9]

GREEN CE, 1978, FRONTIERS PLANT TISS, P414

[10]

JACKSON JA, 1986, PLANT TISSUE CULTURE, P85

← 1 2 3 →