A Change in Nuclear Pore Complex Composition Regulates Cell Differentiation

被引：181

作者：

D'Angelo, Maximilian A. ^{[1
]}

Gomez-Cavazos, J. Sebastian ^{[1
]}

Mei, Arianna ^{[1
]}

Lackner, Daniel H. ^{[1
]}

Hetzer, Martin W. ^{[1
]}

机构：

[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA

来源：

DEVELOPMENTAL CELL | 2012年 / 22卷 / 02期

基金：

美国国家卫生研究院;

关键词：

GENE-EXPRESSION; NUCLEOPORIN; MEMBRANE; TRANSPORT; CHROMATIN; PROTEINS; LOCALIZATION; GLYCOPROTEIN; COMPONENT; CYCLE;

D O I：

10.1016/j.devcel.2011.11.021

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nuclear pore complexes (NPCs) are built from 30 different proteins called nucleoporins or Nups. Previous studies have shown that several Nups exhibit cell-type-specific expression and that mutations in NPC components result in tissue-specific diseases. Here we show that a specific change in NPC composition is required for both myogenic and neuronal differentiation. The transmembrane nucleoporin Nup210 is absent in proliferating myoblasts and embryonic stem cells (ESCs) but becomes expressed and incorporated into NPCs during cell differentiation. Preventing Nup210 production by RNAi blocks myogenesis and the differentiation of ESCs into neuroprogenitors. We found that the addition of Nup210 to NPCs does not affect nuclear transport but is required for the induction of genes that are essential for cell differentiation. Our results identify a single change in NPC composition as an essential step in cell differentiation and establish a role for Nup210 in gene expression regulation and cell fate determination.

引用

页码：446 / 458

页数：13

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