Biomarkers of ocular surface disease using impression cytology

被引:32
作者
Hagan, Suzanne [1 ]
机构
[1] Glasgow Caledonian Univ, Dept Life Sci, Vis Sci, Glasgow G4 0BA, Lanark, Scotland
关键词
biomarkers; conjunctiva; dry eye disease; impression cytology; inflammation; ocular surface; personalized medicine; CONJUNCTIVAL EPITHELIAL-CELLS; FLOW-CYTOMETRIC ANALYSIS; DRY EYE PATIENTS; MESSENGER-RNA EXPRESSION; HLA-DR; TEAR-FILM; INFLAMMATORY MARKERS; CLINICAL-TRIALS; UP-REGULATION; KERATOCONJUNCTIVITIS;
D O I
10.2217/bmm-2017-0124
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Impression cytology (IC) is a technique which permits the retrieval of the outermost layer of ocular surface cells via the use of various types of filters. It is a minimally invasive method of evaluating human conjunctival epithelial cell morphology in the diagnosis of dry eye disease, a common and distressing disorder associated with ageing, contact lens wear, autoimmune disorders and refractive (LASIK) surgery. IC may also be utilized in the diagnosis of other ocular diseases, such as keratoconus and thyroid orbitopathy. More recently, IC has been utilized for the subsequent investigation of gene and protein expression of conjunctival cells in order to identify novel diagnostic biomarkers and to further our understanding of the mechanisms underlying ocular surface disease. This review will therefore examine the literature concerning the role of IC in identifying cellular markers of eye disease, systemic diseases with ocular involvement and potential novel therapeutic targets.
引用
收藏
页码:1135 / 1147
页数:13
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