A new stable and reliable method for labeling nucleic acids of fully replicative viruses

被引：23

作者：

Huang, Li-Li ^{[1
]}

Zhou, Peng ^{[2
]}

Wang, Han-Zhong ^{[3
]}

Zhang, Rui ^{[1
]}

Hao, Jian ^{[1
]}

Xie, Hai-Yan ^{[1
]}

He, Zhi-Ke ^{[2
]}

机构：

[1] Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China

[2] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

[3] Chinese Acad Sci, Wuhan Inst Virol, Beijing 100864, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 18期

基金：

中国国家自然科学基金;

关键词：

ADENOASSOCIATED VIRUS; INFECTION; VISUALIZATION; PARTICLES; TRACKING;

D O I：

10.1039/c2cc17069h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Efficiently labeling nucleic acids of fully replicative viruses is a challenge. In this work, a 'molecular light switch' complex [Ru(phen)(2)(dppz)](2+), where phen = 1,10-phenanthroline and dppz = dipyrido[3,2-a:2',3'-c]phenazine, has been exploringly used to label vaccinia virus nucleic acid. The labeled virions exhibited strong and stable fluorescence and could be imaged at the single-virion level. Moreover, they were fully infectious and can be used to study the behaviors of invasion into their host cells. The method is general and suitable for labeling various DNA viruses.

引用

页码：2424 / 2426

页数：3

共 16 条

[1] Imaging poliovirus entry in live cells [J].

Brandenburg, Boerries ;

Lee, Lily Y. ;

Lakadamyali, Melike ;

Rust, Michael J. ;

Zhuang, Xiaowei ;

Hogle, James M. .

PLOS BIOLOGY, 2007, 5 (07) :1543-1555

[2] Site-specific labeling of enveloped viruses with quantum dots for single virus tracking [J].

Joo, Kye-Il ;

Lei, Yuning ;

Lee, Chi-Lin ;

Lo, Jonathon ;

Xie, Jiansong ;

Hamm-Alvarez, Sarah F. ;

Wang, Pin .

ACS NANO, 2008, 2 (08) :1553-1562

[3] Enhanced Real-Time Monitoring of Adeno-Associated Virus Trafficking by Virus-Quantum Dot Conjugates [J].

Joo, Kye-Il ;

Fang, Yun ;

Liu, Yarong ;

Xiao, Liang ;

Gu, Zhen ;

Tai, April ;

Lee, Chi-Lin ;

Tang, Yi ;

Wang, Pin .

ACS NANO, 2011, 5 (05) :3523-3535

[4] Double-labeled rabies virus: Live tracking of enveloped virus transport [J].

Klingen, Yvonne ;

Conzelmann, Karl-Klaus ;

Finke, Stefan .

JOURNAL OF VIROLOGY, 2008, 82 (01) :237-245

[5] Labeling of capsid proteins and genomic RNA of human rhinovirus with two different fluorescent dyes for selective detection by capillary electrophoresis [J].

Kremser, L ;

Petsch, M ;

Blaas, D ;

Kenndler, E .

ANALYTICAL CHEMISTRY, 2004, 76 (24) :7360-7365

[6] Atomic force microscopy investigation of vaccinia virus structure [J].

Kumetsov, Y. ;

Gershon, P. D. ;

McPherson, A. .

JOURNAL OF VIROLOGY, 2008, 82 (15) :7551-7566

[7] Visualizing infection of individual influenza viruses [J].

Lakadamyali, M ;

Rust, MJ ;

Babcock, HP ;

Zhuang, XW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) :9280-9285

[8] Double-labelled HIV-1 particles for study of virus-cell interaction [J].

Lampe, Marko ;

Briggs, John A. G. ;

Endress, Thomas ;

Glass, Baerbel ;

Riegelsberger, Stefan ;

Kraeusslich, Hans-Georg ;

Lamb, Don C. ;

Braeuchle, Christoph ;

Mueller, Barbara .

VIROLOGY, 2007, 360 (01) :92-104

[9] Establishment of cell-based reporter system for diagnosis of poxvirus infection [J].

Levy, Orly ;

Oron, Chaya ;

Paran, Nir ;

Keysary, Avi ;

Israeli, Ofir ;

Yitzhaki, Shmuel ;

Olshevsky, Udy .

JOURNAL OF VIROLOGICAL METHODS, 2010, 167 (01) :23-30

[10] First observation of the key intermediate in the ''light-switch'' mechanism of [Ru(phen)(2)dppz](2+) [J].

Olson, EJC ;

Hu, D ;

Hormann, A ;

Jonkman, AM ;

Arkin, MR ;

Stemp, EDA ;

Barton, JK ;

Barbara, PF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (47) :11458-11467

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