Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses

被引：24
作者：
Wu, Peipei [1 ,2 ,3 ]
Lu, Jihu [1 ,2 ,3 ]
Zhang, Xuehua [1 ,2 ,3 ]
Mei, Mei [1 ,2 ,3 ]
Feng, Lei [1 ,2 ,3 ]
Peng, Daxin [3 ,4 ]
Hou, Jibo [1 ,2 ,3 ]
Kang, Sang-Moo [5 ]
Liu, Xiufan [3 ,4 ]
Tang, Yinghua [1 ,2 ,3 ]
机构：
[1] Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing, Jiangsu, Peoples R China
[2] Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Nanjing, Jiangsu, Peoples R China
[3] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China
[4] Yangzhou Univ, Coll Vet Med, Anim Infect Dis Lab, Yangzhou, Jiangsu, Peoples R China
[5] Georgia State Univ, Inst Biomed Sci, Ctr Inflammat Immun & Infect, Atlanta, GA 30303 USA
来源：
FRONTIERS IN IMMUNOLOGY | 2017年 / 8卷
关键词：
H5 subtype influenza virus; vaccine; consensus; virus-like particles; broad protection; AVIAN-INFLUENZA; INACTIVATED VACCINE; DNA VACCINES; IMMUNOGENICITY; PATHOGENICITY; EMERGENCE; RESPONSES; EFFICACY;
D O I：
10.3389/fimmu.2017.01649
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The H5 subtype highly pathogenic avian influenza (HPAI) virus is one of the greatest threats to global poultry industry. To develop broadly protective H5 subunit vaccine, a recombinant consensus HA sequence (rHA) was constructed and expressed in virus-like particles (rHA VLPs) in the baculovirus-insect cell system. The efficacy of the rHA VLPs vaccine with or without immunopotentiator (CVCVA5) was assessed in chickens. Compared to the commercial Re6 or Re6-CVCVA5 vaccines, single dose immunization of chickens with rHA VLPs or rHA-CVCVA5 vaccines induced higher levels of serum hemagglutinin inhibition titers and neutralization titers, mucosal antibodies, IFN-gamma and IL-4 cytokines in sera, and cytotoxic T lymphocyte responses. The rHA VLPs vaccine was superior to the commercial Re6 vaccine in conferring cross-protection against different clades of H5 subtype viruses. This study reports that H5 subtype consensus HA VLP single dose vaccination provides broad protection against HPAI virus in chickens.
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页数：11
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