Determination of human insulin and its six therapeutic analogues by capillary electrophoresis - mass spectrometry

被引：12

作者：

Hamidli, Narmin ^{[1
]}

Pajaziti, Blerta ^{[1
]}

Andrasi, Melinda ^{[1
]}

Nagy, Cynthia ^{[1
]}

Gaspar, Attila ^{[1
]}

机构：

[1] Univ Debrecen, Dept Inorgan & Analyt Chem, Egyet Ter 1, H-4032 Debrecen, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY A | 2022年 / 1678卷

关键词：

Insulin; Insulin analogues; Therapeutics; Capillary electrophoresis; Mass spectrometry; ZONE-ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; PROTEINS; SEPARATION; PERFORMANCE; STABILITY; PEPTIDES; CZE;

D O I：

10.1016/j.chroma.2022.463351

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, human insulin and its 6 analogues were separated and determined using CZE-MS. Three different capillaries (bare fused silica, successive multiple ionic-polymer layer (SMIL) and static linear polyacrylamide (LPA) coated) were compared based on their separation performances in their optimal operating conditions. Coated capillaries demonstrated slightly better separation of the components, although some components showed wide, distorted peaks. The highest plate number could be obtained in the SMIL capillary (192 000/m). For UV and ESI-MS detection relatively similar LOD values were obtained (0.3-1.2 mg/L and 1.0-3.4 mg/L, respectively). The application of MS detection provided useful structural information and unambiguous identification for insulins having similar or the same molecular mass. This work is considered to be important not only for the investigation of insulins but also for its potential contribution to the top-down analysis of proteins using CE-MS. (C) 2022 The Authors. Published by Elsevier B.V.

引用

页数：9

共 39 条

[1] A new insulin immunoassay specific for the rapid-acting insulin analog, insulin aspart, suitable for bioavailability, bioequivalence, and pharmacokinetic studies [J].

Andersen, L ;

Jorgensen, PN ;

Jensen, LB ;

Walsh, D .

CLINICAL BIOCHEMISTRY, 2000, 33 (08) :627-633

[2] Determination of deamidated isoforms of human insulin using capillary electrophoresis [J].

Andrasi, M. ;

Pajaziti, B. ;

Sipos, B. ;

Nagy, C. ;

Hamidli, N. ;

Gaspar, A. .

JOURNAL OF CHROMATOGRAPHY A, 2020, 1626

[3]

[Anonymous], 2018, MON INS ASP 2084 INS, V9th

[4] Stability and effectiveness of linear polyacrylamide capillary coating to suppress EOF in acidic media in the presence of surfactants, ionic liquids and organic modifiers [J].

Beneito-Cambra, Miriam ;

Anres, Philippe ;

Vial, Jerome ;

Gareil, Pierre ;

Delaunay, Nathalie .

TALANTA, 2016, 150 :546-552

[5] Rapid mass spectrometric determination of disulfide connectivity in peptides and proteins [J].

Bhattacharyya, Moitrayee ;

Gupta, Kallol ;

Gowd, Konkallu Hanumae ;

Balaram, Padmanabhan .

MOLECULAR BIOSYSTEMS, 2013, 9 (06) :1340-1350

[6] High-Resolution Capillary Zone Electrophoresis with Mass Spectrometry Peptide Mapping of Therapeutic Proteins: Improved Separation with Mixed Aqueous-Aprotic Dipolar Solvents (N,N-Dimethylacetamide and N,N-Dimethylformamide) as the Background Electrolyte [J].

Dada, Oluwatosin O. ;

Zhao, Yimeng ;

Jaya, Nomalie ;

Salas-Solano, Oscar .

ANALYTICAL CHEMISTRY, 2017, 89 (21) :11227-11235

[7]

Donner T., 2000, Insulin - Pharmacology, Therapeutic Regimens, and Principles of Intensive Insulin Therapy

[8]

Eriksson H.J.C., 2004, THESIS U GRONINGEN G

[9] Analysis of intact proteins with capillary zone electrophoresis coupled to mass spectromery using uncoated and coated capillaries [J].

Hamidli, N. ;

Andrasi, M. ;

Nagy, C. ;

Gaspar, A. .

JOURNAL OF CHROMATOGRAPHY A, 2021, 1654

[10] Thickness and morphology of polyelectrolyte coatings on silica surfaces before and after protein exposure studied by atomic force microscopy [J].

Haselberg, Rob ;

Flesch, Frits M. ;

Boerke, Arjan ;

Somsen, Govert W. .

ANALYTICA CHIMICA ACTA, 2013, 779 :90-95

← 1 2 3 4 →