Gold nanorods-based FRET assay for sensitive detection of Pb2+ using 8-17DNAzyme

被引：36
作者：
Wang, Lin [1 ]
Jin, Yan [1 ]
Deng, Jing [1 ]
Chen, Guozhen [1 ]
机构：
[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Minist Educ,Key Lab Appl Surface & Colloid Chem, Xian 710062, Peoples R China
来源：
ANALYST | 2011年 / 136卷 / 24期
基金：
中国国家自然科学基金;
关键词：
ATOMIC-ABSORPTION SPECTROSCOPY; ANODIC-STRIPPING VOLTAMMETRY; PLASMA-MASS SPECTROMETRY; FLUORESCENCE POLARIZATION; METAL-IONS; LEAD IONS; DNA; DNAZYME; NANOPARTICLES; ELECTRODE;
D O I：
10.1039/c1an15783c
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this paper, we have reported a sensitive assay for fluorescence "turn-on" detection of Pb2+ in aqueous solutions based on FRET between gold nanorods (GNRs) and the FAM-labeled substrate strand of 8-17DNAzyme. The fluorescence of the FAM-labeled substrate strand is quenched when 8-17DNAzyme is adsorbed on GNRs surface through electrostatic interaction. In the presence of lead ions, the fluorescence is restored due to the decrease of FRET efficiency caused by the specific cleavage of the FAM-labeled substrate strand by the enzyme, which weakens the electrostatic interaction between the GNRs and short FAM-labeled DNA fragment. The interference of eleven common metal ions has been tested, indicating that Pb2+ can be selectively detected. This method exhibits a high sensitivity for Pb2+ with a detection limit of 61.8 pM and a linear range from 0.1 nM to 100 nM. It is a simple, sensitive, and selective method for Pb2+ detection. Moreover, this sensing system obtained satisfying results for Pb2+ detection in tap water samples.
引用
收藏
页码：5169 / 5174
页数：6
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