Porous graphitic carbon as a stationary phase in HPLC: Theory and applications

被引：140

作者：

Pereira, Luisa ^{[1
]}

机构：

[1] Thermo Fisher Sci, Runcorn WA7 1PR, Cheshire, England

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2008年 / 31卷 / 11-12期

关键词：

electronic interaction chromatography; hypercarb; isomers; polar retention effect on graphite; polar solutes; retention mechanisms;

D O I：

10.1080/10826070802126429

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper gives an overview of the properties and behaviour of porous graphitic carbon (PGC) as a stationary phase in HPLC. Key applications areas that illustrate the capabilities of this chromatographic material to solve problem separations are reviewed. The retention mechanisms on this stationary phase for hydrophobic and polar solutes are described. PGCs' physical and chemical properties and the development of this unique chromatographic material are discussed. Over 140 references of applications of PGC in pharmaceutical, environmental, food safety, natural products, biochemical, clinical, and chemical warfare analysis are reviewed covering a range of solute properties.

引用

页码：1687 / 1731

页数：45

共 176 条

[1] Separation of underivatized small peptides on a porous graphitic carbon column by ion-pair chromatography and evaporative light scattering detection [J].

Adoubel, AA ;

Guenu, S ;

Elfakir, C ;

Dreux, M .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2000, 23 (16) :2433-2446

[2] A selective method for the determination of benzo[a]pyrene in soil using porous graphitic carbon liquid chromatography columns [J].

Al-Haddad, A .

TALANTA, 2003, 59 (04) :845-848

[3]

ALTORFER H, 2002, PHARMAEUROPA, V14, P637

[4] Quantification of sugars and sugar phosphates in Arabidopsis thaliana tissues using porous graphitic carbon liquid chromatography-electrospray ionization mass spectrometry [J].

Antonio, Carla ;

Larson, Tony ;

Gilday, Alison ;

Graham, Ian ;

Bergstroem, Ed ;

Thomas-Oates, Jane .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1172 (02) :170-178

[5] Characterisation of ι-carrageenans oligosaccharides with high-performance liquid chromatography coupled with evaporative light scattering detection [J].

Antonopoulos, A ;

Favetta, P ;

Lafosse, M ;

Helbert, W .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1059 (1-2) :83-87

[6] Comparative analysis of enzymatically digested κ-carrageenans, using liquid chromatography on ion-exchange and porous graphitic carbon columns coupled to an evaporative light scattering detector [J].

Antonopoulos, A ;

Herbreteau, B ;

Lafosse, M ;

Helbert, W .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1023 (02) :231-238

[7] On-line liquid chromatography-electrospray ionisation mass spectrometry for κ-carrageenan oligosaccharides with a porous graphitic carbon column [J].

Antonopoulos, Aristotelis ;

Favetta, Patrick ;

Helbert, William ;

Lafosse, Michel .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1147 (01) :37-41

[8] Separation and determination of synthetic corticosteroids in bovine liver by LC-ion-trap-MS-MS on porous graphite [J].

Baiocchi, C ;

Brussino, M ;

Pazzi, M ;

Medana, C ;

Marini, C ;

Genta, E .

CHROMATOGRAPHIA, 2003, 58 (1-2) :11-14

[9]

Barnes KA, 1997, RAPID COMMUN MASS SP, V11, P159, DOI 10.1002/(SICI)1097-0231(19970131)11:2<159::AID-RCM730>3.0.CO

[10]

2-O

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