Swollenin pre-conditioning: optimization studies and application aiming at d-lactic acid production from sugarcane bagasse

被引:1
|
作者
Modesto, Luiz Felipe [1 ]
Mendez, Johanna [1 ,2 ]
Wischral, Daiana [1 ]
Pereira, Nei [1 ]
机构
[1] Univ Fed Rio de Janeiro, Sch Chem, Dept Biochem Engn, Labs Bioproc Dev, Ave Horacio Macedo 2030,Bloco E, BR-21949900 Rio De Janeiro, RJ, Brazil
[2] Univ Costa Rica, Escuela Ingn Ind, Inst Invest Ingn, Ciudad Univ Rodrigo Facio, San Pedro, Montes De Oca, Costa Rica
关键词
Enzymatic hydrolysis; lactic acid; lignocellulosic biomass; pre-conditioning; swollenin; sugarcane bagasse; ENZYMATIC-HYDROLYSIS; FERMENTATION; WASTE;
D O I
10.1080/00986445.2018.1477762
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The enzymatic hydrolysis still stands as a challenge regarding the use of renewable feedstocks, as well as of lignocellulosic materials. The present work reports optimization of swollenin addition to pretreated sugarcane bagasse, in a step prior to the enzymatic hydrolysis, named pre-conditioning. Also, it describes further fermentation of the hydrolyzed material to d-lactic acid. Trichoderma harzianum enzymatic cocktail was produced from pretreated sugarcane bagasse, while swollenin was produced using Aspergillus niger as a host for recombinant protein expression. For pre-conditioning optimization, a Central Composite Rotatable Design was applied and validated. The optimized conditions were used to produce the hydrolysate that was fermented to D-lactic acid by Lactobacillus coryniformis subsp. torquens. It could be observed that pre-conditioning effectively leads to improvements in glucose release: 62% of hydrolysis efficiency. Besides, it was possible to use the produced hydrolysate to obtain d-lactic acid, with a yield of 1.34 g/g and a productivity of 1.59 g/L h(-1).
引用
收藏
页码:149 / 157
页数:9
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