Silver Nanoparticles as a Glucose Oxidase Immobilization Matrix for Amperometric Glucose Biosensor Construction

被引：0

作者：

Hsu, Fu-Yin ^{[1
,2
]}

Yu, Ding-Syuan ^{[1
,2
]}

Chang, Jung-Che ^{[2
]}

Chuang, Chia-Lin ^{[3
]}

机构：

[1] Natl Taiwan Ocean Univ, Dept Life Sci, Chilung, Taiwan

[2] Natl Taiwan Ocean Univ, Inst Biosci & Biotechnol, Chilung, Taiwan

[3] Ching Kuo Inst Management & Hlth, Dept Appl Cosmet Sci, Chilung, Taiwan

来源：

JOURNAL OF THE CHINESE CHEMICAL SOCIETY | 2011年 / 58卷 / 06期

关键词：

Silver nanoparticles; Glucose oxidase; Immobilization; Biosensor; GOLD NANOPARTICLES; CARBON NANOTUBES; ELECTRODE; CHITOSAN; LAYER;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Immobilized biomolecules are widely applied in medical, food and environmental fields. In this paper, glucose oxidase (GOD) was covalently immobilized on the surface of thiol-modified silver nanoparticles (AgNPs) to fabricate GOD-AgNP bioconjugate complexes. GOD-AgNP complex were more stable at high temperature and in high pH environments than the free GOD in solution. Additionally, the fabricated Carbon rod/GOD-AgNPs/Nafion/Chitosan electrode exhibited a rapid response and a linear calibration range from 0.5 mM to 6 mM for the detection of glucose. The Nafion/Chitosan coating could eliminate common interferents, such as uric acid and acetaminophen. As a conclusion, the experimental results indicated that the resulting modified electrode possesses an excellent response performance on glucose.

引用

收藏

页码：756 / 760

页数：5

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