Extraction and identification of antioxidative components of Hsian-tsao (Mesona procumbens Hemsl.)

被引：49

作者：

Hung, CY ^{[1
]}

Yen, GC ^{[1
]}

机构：

[1] Natl Chunghsing Univ, Dept Food Sci, Taichung 402, Taiwan

来源：

LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY | 2001年 / 34卷 / 05期

关键词：

Mesona procumbens Hemsl; antioxidant activity; stigmasterol; beta-sitosterol; oleanolic acid; ursolic acid;

D O I：

10.1006/fstl.2001.0775

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The antioxidative activities of various solvent extracts of Hsian-tsao (Mesona procumbens Hemsl) were investigated. The results showed that for the same concentration 0.2 g/kg, the antioxidative activities of various Hsian-tsao extracts, especially the ethyl acetate extract, which exhibited 93% inhibition of peroxidation of linoleic acid, were greater than those of BHA and alpha-tocopherol. When the ethyl acetate extract of Hsian-tsao (EEHT) was separated into nine fractions by silica gel chromatography fraction II showed high yield and high antioxidative activity. Fraction II was further separated into five subfractions using silica gel chromatography. Subfraction IIC was then isolated by preparative HPLC equipped with a silica column and four components were identified as stigmasterol, beta-sitosterol, oleanolic acid, and ursolic acid by UV, EI-MS, H-1-, and C-13-ArMR. The descending order of antioxidative activity for those compounds was: EEhT > BHA > alpha-tocopherol > ursolic acid > oleanolic acid > beta-sitosterol > stigmasterol.

引用

页码：306 / 311

页数：6

共 14 条

[1]

BALANEHRU S, 1992, BIOCHEM INT, V28, P735

[2]

BALANEHRU S, 1991, BIOCHEM INT, V24, P981

[3] EFFECTS OF ROSEMARY EXTRACTS AND MAJOR CONSTITUENTS ON LIPID OXIDATION AND SOYBEAN LIPOXYGENASE ACTIVITY [J].

CHEN, QY ;

SHI, H ;

HO, CT .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1992, 69 (10) :999-1002

[4] GYCOMOSIDE-I - A NEW DAMMARANE SAPONIN FROM GYNOSTEMMA-COMPRESSUM [J].

DING, SL ;

ZHU, ZY .

PLANTA MEDICA, 1993, 59 (04) :373-375

[5] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTRA OF SOME C-19-HYDROXY, C-5,6 EPOXY, C-24 ETHYL, AND C-19-NORSTEROIDS [J].

HOLLAND, HL ;

DIAKOW, PRP ;

TAYLOR, GJ .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1978, 56 (24) :3121-3127

[6]

ITO N, 1983, JNCI-J NATL CANCER I, V70, P343

[7] Anti-AIDS agents. 30. Anti-HIV activity of oleanolic acid, pomolic acid, and structurally related triterpenoids [J].

Kashiwada, Y ;

Wang, HK ;

Nagao, T ;

Kitanaka, S ;

Yasuda, I ;

Fujioka, T ;

Yamagishi, T ;

Cosentino, LM ;

Kozuka, M ;

Okabe, K ;

Ikeshiro, Y ;

Hu, CQ ;

Yeh, E ;

Lee, KH .

JOURNAL OF NATURAL PRODUCTS, 1998, 61 (09) :1090-1095

[8]

KAZUKI H, 1996, Patent No. 083551

[9] STUDIES ON THE CONSTITUENTS OF FORMOSAN GENTIANACEOUS PLANTS .9. XANTHONES FROM FORMOSAN GENTIANACEOUS PLANTS [J].

LIN, CN ;

CHUNG, MI ;

GAN, KH ;

CHIANG, JR .

PHYTOCHEMISTRY, 1987, 26 (08) :2381-2384

[10]

Mitsuda H., 1966, J JAPAN SOC FOOD NUT, V19, P210

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