All-trans retinoic acid induces oxidative phosphorylation and mitochondria biogenesis in adipocytes

被引：84

作者：

Tourniaire, Franck ^{[1
,2
,3
]}

Musinovic, Hana ^{[5
,6
]}

Gouranton, Erwan ^{[1
,2
,3
]}

Astier, Julien ^{[1
,2
,3
]}

Marcotorchino, Julie ^{[1
,2
,3
]}

Arreguin, Andrea ^{[5
,6
]}

Bernot, Denis ^{[4
]}

Palou, Andreu ^{[5
,6
]}

Bonet, M. Luisa ^{[5
,6
]}

Ribot, Joan ^{[5
,6
]}

Landrier, Jean-Francois ^{[1
,2
]}

机构：

[1] INRA, UMR 1260, F-13385 Marseille, France

[2] INSERM, UMR Nutr Obes & Risque Thrombot 1062, F-13385 Marseille, France

[3] Aix Marseille Univ, Fac Med, F-13385 Marseille, France

[4] CHU La Timone, AP HM, F-13385 Marseille, France

[5] Univ Illes Balears, Lab Mol Biol Nutr & Biotechnol, Nutrigen Grp, Palma De Mallorca, Spain

[6] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Palma De Mallorca, Spain

来源：

JOURNAL OF LIPID RESEARCH | 2015年 / 56卷 / 06期

关键词：

vitamin A; adipose tissue; browning; VITAMIN-A SUPPLEMENTATION; ADIPOSE-TISSUE BIOLOGY; ACTIVATED-RECEPTOR; GENE-EXPRESSION; LEPTIN EXPRESSION; BROWN FAT; PGC-1; COACTIVATORS; INSULIN-RESISTANCE; MATURE ADIPOCYTES; LIPID OXIDATION;

D O I：

10.1194/jlr.M053652

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A positive effect of all-trans retinoic acid (ATRA) on white adipose tissue (WAT) oxidative and thermogenic capacity has been described and linked to an in vivo fat-lowering effect of ATRA in mice. However, little is known about the effects of ATRA on mitochondria in white fat. Our objective has been to characterize the effect of ATRA on mitochondria biogenesis and oxidative phosphorylation (OXPHOS) capacity in mature white adipocytes. Transcriptome analysis, oxygraphy, analysis of mitochondrial DNA (mtDNA), and flow cytometry-based analysis of mitochondria density were performed in mature 3T3-L1 adipocytes after 24 h incubation with ATRA (2 mu M) or vehicle. Selected genes linked to mitochondria biogenesis and function and mitochondria immunostaining were analyzed in WAT tissues of ATRA-treated as compared with vehicle-treated mice. ATRA upregulated the expression of a large set of genes linked to mtDNA replication and transcription, mitochondrial biogenesis, and OXPHOS in adipocytes, as indicated by transcriptome analysis. Oxygen consumption rate, mtDNA content, and staining of mitochondria were increased in the ATRA-treated adipocytes. Similar results were obtained in WAT depots of ATRA-treated mice. We conclude that ATRA impacts mitochondria in adipocytes, leading to increased OXPHOS capacity and mitochondrial content in these cells.

引用

页码：1100 / 1109

页数：10

共 45 条

[1] Both retinoic-acid-receptor- and retinoid-X-receptor-dependent signalling pathways mediate the induction of the brown-adipose-tissue-uncoupling-protein-1 gene by retinoids [J].