Two-photon excited polarized fluorescence in NADH in methanol/water solutions

被引：0
|
作者：
Gorbunova, I. A. [1 ,2 ]
Bezverkhnii, N. O. [1 ,3 ]
Zhikhoreva, A. A. [1 ]
Beltukov, Y. M. [1 ]
Sasin, M. E. [1 ]
Rubayo-Soneira, J. [4 ]
Vasyutinskii, O. S. [1 ]
机构：
[1] Ioffe Inst, 26 Polytekhnicheskaya, St Petersburg, Russia
[2] St Petersburg Acad Univ, 8-3 Khlopina, St Petersburg, Russia
[3] Peter Great St Petersburg Polytech Univ, 29 Polytechnicheskaya, St Petersburg, Russia
[4] Univ La Habana, Inst Super Tecnol & Ciencias Aplicadas, Havana, Cuba
来源：
ADVANCES IN MICROSCOPIC IMAGING II | 2019年 / 11076卷
基金：
俄罗斯基础研究基金会;
关键词：
biological coenzyme; NADH; adenine; nicotinamide; polarized fluorescence; spectroscopy; TCSPC; solutions viscosity; INTRACELLULAR NADH; DYNAMICS; CONFORMATION; EXCITATION; DECAY;
D O I：
10.1117/12.2536641
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We studied polarized fluorescence in biological coenzyme NADH in water-methanol solutions upon two-photon excitation with femtosecond laser pulses at 720 nm. The polarized fluorescence decay was recorded by a time correlated single photon counting (TCSPC) system. Fluorescence decay times, rotational diffusion time, fluorescence anisotropy, and the ratio of two pre-exponential factors have been determined and studied as a function of methanol concentration. The results obtained were interpreted on the basis of a model of NADH denaturation processes in solutions and can be used for modeling of NADH binding with various dehydrogenases in living cells.
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页数：6
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