Insulin-Loaded SLN Prepared with the Emulsion Dilution Technique: In Vivo Tracking of Nanoparticles after Oral Administration to Rats

被引：16

作者：

Trotta, M. ^{[1
]}

Carlotti, M. E. ^{[1
]}

Gallarate, M. ^{[1
]}

Zara, G. P. ^{[2
]}

Muntoni, E. ^{[2
]}

Battaglia, L. ^{[1
]}

机构：

[1] Univ Turin, Dipartimento Sci & Tecnol Farm, Turin, Italy

[2] Univ Turin, Dipartimento Anat Farmacol & Med Legale, Turin, Italy

来源：

JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY | 2011年 / 32卷 / 07期

关键词：

FITC-insulin; intestinal uptake; SLN; SOLID LIPID NANOPARTICLES; PARTICLE-SIZE; MICROSPHERES; PERFORMANCE; NANOSPHERES; DELIVERY; CARRIERS;

D O I：

10.1080/01932691.2010.488497

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Insulin-loaded solid lipid nanoparticles (SLN) were prepared according to a solvent dilution method from O/W emulsions using isovaleric acid as organic phase. Insulin was derivatized with fluorescein isothyocianate (FITC) obtaining a fluorescent marker to be used in in vivo experiments. FITC-insulin and native insulin-loaded SLN were quite similar with regard to their mean sizes and encapsulation efficiency. SLN intestinal uptake was then investigated administering FITC-insulin loaded SLN on healthy male Wistar rats. Significant drug accumulation within intestinal lymphatic system was recovered, but the immune system seems to play an important role in SLN degradation: further studies are necessary to improve the results on blood glucose level.

引用

页码：1041 / 1045

页数：5

共 28 条

[1] Visualization of Insulin-Loaded Nanocapsules: In Vitro and in Vivo Studies After Oral Administration to Rats
Huguette Pinto-Alphandary
Malam Aboubakar
Danielle Jaillard
Patrick Couvreur
Christine Vauthier
Pharmaceutical Research, 2003, 20 : 1071 - 1084
[2] Visualization of insulin-loaded nanocapsules:: in vitro and in vivo studies after oral administration to rats
Pinto-Alphandary, H
Aboubakar, M
Jaillard, D
Couvreur, P
Vauthier, C
PHARMACEUTICAL RESEARCH, 2003, 20 (07) : 1071 - 1084
[3] Absorption and efficiency of insulin after oral administration of insulin-loaded nanocapsules in diabetic rats
Cournarie, F
Auchere, D
Chevenne, D
Lacour, B
Seiller, M
Vauthier, C
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2002, 242 (1-2) : 325 - 328
[4] Insulin-loaded nanocapsules for oral administration: In vitro and in vivo investigation
Aboubakar, M
Couvreur, P
Pinto-Alphandary, H
Gouritin, B
Lacour, B
Farinotti, R
Puisieux, F
Vauthier, C
DRUG DEVELOPMENT RESEARCH, 2000, 49 (02) : 109 - 117
[5] Preparation and characterization of insulin-loaded bioadhesive PLGA nanoparticles for oral administration
Zhang, Xiaoyan
Sun, Muzhen
Zheng, Aiping
Cao, Deying
Bi, Yunqi
Sun, Jianxu
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2012, 45 (05) : 632 - 638
[6] In vivo evaluation of biochemical efficacy of prepared insulin-loaded nanoliposomes for oral delivery
Gharib, Amir
Faezizadeh, Zohreh
CLINICAL BIOCHEMISTRY, 2011, 44 (13) : S218 - S218
[7] In vivo evaluation of physiological efficacy of insulin-loaded nanoliposomes prepared for oral delivery
Gharib, Amir
Faezizadeh, Zohreh
PHYSIOLOGY AND PHARMACOLOGY, 2011, 15 (01): : 57 - 65
[8] Enhanced oral bioavailability of insulin-loaded solid lipid nanoparticles: pharmacokinetic bioavailability of insulin-loaded solid lipid nanoparticles in diabetic rats
Ansari, Mohammad Javed
Anwer, Md. Khalid
Jamil, Shahid
Al-Shdefat, Ramadan
Ali, Bahaa E.
Ahmad, Mohammad Muqtader
Ansari, Mohammad Nazam
DRUG DELIVERY, 2016, 23 (06) : 1972 - 1979
[9] Prolonged hypoglycemic effect of insulin-loaded polybutylcyanoacrylate nanoparticles after pulmonary administration to normal rats
Zhang, Q
Shen, ZC
Nagai, T
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 218 (1-2) : 75 - 80
[10] Insulin-Loaded PLGA Nanoparticles for Oral Administration: An In Vitro Physico-Chemical Characterization
Santander-Ortega, M. J.
Bastos-Gonzalez, D.
Ortega-Vinuesa, J. L.
Alonso, M. J.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2009, 5 (01) : 45 - 53

← 1 2 3 →