Vascular Endothelial-Breast Epithelial Cell Coculture Model Created from 3D Cell Structures

Endothelial cell interactions with normal and cancerous breast epithelial cells have been widely studied in tissue growth and development, as well as in angiogenesis and metastasis. Despite the understanding that 3D multicellular architecture is critical to the cell phenotype, 3D vascular structures have not yet been cocultured with 3D breast spheroids in vitro. The objective of this study was therefore to create a hierarchical, multiscale model of vascular endothelial-breast epithelial cell interactions in which both cell types were assembled into their 3D architectures. The model was successfully fabricated by adding preformed breast spheroids onto preformed endothelial tube-like networks. Through this model, we observed that breast spheroids maintain vascular tube-like networks. Over time, breast epithelial cells migrate out of the spheroid structure along the endothelial networks. This research shows that 3D cell structures serve as an important building block for creating multicellular coculture models to study physiologically relevant cell cell interactions.