miR-194 is a negative regulator of GEF-H1 pathway in melanoma

被引：18

作者：

Guo, Bingyu ^{[1
]}

Hui, Qiang ^{[1
]}

Zhang, Yu ^{[1
]}

Chang, Peng ^{[1
]}

Tao, Kai ^{[1
]}

机构：

[1] Shenyang Mil Reg, Gen Hosp, Plast & Reconstruct Surg, 83 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China

来源：

ONCOLOGY REPORTS | 2016年 / 36卷 / 04期

关键词：

GEF-H1; melanoma; prognosis; proliferation; metastasis; NUCLEOTIDE EXCHANGE FACTOR; CELL-PROLIFERATION; CANCER-CELLS; SIGNALING PATHWAY; GASTRIC-CANCER; SUPPRESSES METASTASIS; COLORECTAL-CANCER; RHOGEF GEF-H1; CYCLIN D1; RT-PCR;

D O I：

10.3892/or.2016.5020

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The incidence and associated mortality of melanoma continues to increase worldwide. At present, there is no curative therapy for advanced stage of melanoma. It is necessary to find new indicators of prognosis and therapeutic targets. Increasing evidence shows that miRNA can provide potential candidate biomarkers for melanoma. and therapeutic targets. GEF-H1, a regulator of RhoA, as oncogenic driver in melanoma, promotes the growth and invasion of melanoma. miR-194 is a tumor-suppressor gene in multiple tumors, such as bladder and non-small cell lung cancer, and clear cell renal cell carcinoma. In the present study, we demonstrated that GEF-H1 serves as target of miR-194. Overexpression of miR-194 downregulates the GEF-H1/RhoA pathway, inhibits melanoma cancer cell proliferation and metastasis. Furthermore, miR-194 expression is negatively associated with tumor-node-metastasis (TNM) stages. Briefly, our findings provided new theoretical basis for melanoma treatment.

引用

页码：2412 / 2420

页数：9

共 48 条

[1] Effect of Ursolic Acid on MAPK in Cyclin D1 Signaling and RING-Type E3 Ligase (SCF E3s) in Two Endometrial Cancer Cell Lines [J].

Achiwa, Yumiko ;

Hasegawa, Kiyoshi ;

Udagawa, Yasuhiro .

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2013, 65 (07) :1026-1033

[2] Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition [J].

Aijaz, S ;

D'Atri, F ;

Citi, S ;

Balda, MS ;

Matter, K .

DEVELOPMENTAL CELL, 2005, 8 (05) :777-786

[3] Future perspectives in melanoma research: meeting report from the "Melanoma Bridge": Napoli, December 3rd-6th 2014 [J].

Ascierto, Paolo A. ;

Atkins, Michael ;

Bifulco, Carlo ;

Botti, Gerardo ;

Cochran, Alistair ;

Davies, Michael ;

Demaria, Sandra ;

Dummer, Reinhard ;

Ferrone, Soldano ;

Formenti, Silvia ;

Gajewski, Thomas F. ;

Garbe, Claus ;

Khleif, Samir ;

Kiessling, Rolf ;

Lo, Roger ;

Lorigan, Paul ;

Mc Arthur, Grant ;

Masucci, Giuseppe ;

Melero, Ignacio ;

Mihm, Martin ;

Palmieri, Giuseppe ;

Parmiani, Giorgio ;

Puzanov, Igor ;

Romero, Pedro ;

Schilling, Bastian ;

Seliger, Barbara ;

Stroncek, David ;

Taube, Janis ;

Tomei, Sara ;

Zarour, Hassane M. ;

Testori, Alessandro ;

Wang, Ena ;

Galon, Jerome ;

Ciliberto, Gennaro ;

Mozzillo, Nicola ;

Marincola, Francesco M. ;

Thurin, Magdalena .

JOURNAL OF TRANSLATIONAL MEDICINE, 2015, 13

[4] Rho GTPases:: potential candidates for anticancer therapy [J].

Aznar, S ;

Fernández-Valerón, P ;

Espina, C ;

Lacal, JC .

CANCER LETTERS, 2004, 206 (02) :181-191

[5] Final Version of 2009 AJCC Melanoma Staging and Classification [J].

Balch, Charles M. ;

Gershenwald, Jeffrey E. ;

Soong, Seng-jaw ;

Thompson, John F. ;

Atkins, Michael B. ;

Byrd, David R. ;

Buzaid, Antonio C. ;

Cochran, Alistair J. ;

Coit, Daniel G. ;

Ding, Shouluan ;

Eggermont, Alexander M. ;

Flaherty, Keith T. ;

Gimotty, Phyllis A. ;

Kirkwood, John M. ;

McMasters, Kelly M. ;

Mihm, Martin C., Jr. ;

Morton, Donald L. ;

Ross, Merrick I. ;

Sober, Arthur J. ;

Sondak, Vernon K. .

JOURNAL OF CLINICAL ONCOLOGY, 2009, 27 (36) :6199-6206

[6] Circulating levels of the miRNAs, miR-194, and miR-29b, as clinically useful biomarkers for colorectal cancer [J].

Basati, Gholam ;

Razavi, Amirnader Emami ;

Pakzad, Iraj ;

Malayeri, Fardin Ali .

TUMOR BIOLOGY, 2016, 37 (02) :1781-1788

[7] miR in melanoma development: miRNAs and acquired hallmarks of cancer in melanoma [J].

Bennett, Paige E. ;

Bemis, Lynne ;

Norris, David A. ;

Shellman, Yiqun G. .

PHYSIOLOGICAL GENOMICS, 2013, 45 (22) :1049-1059

[8] RalB regulates contractility-driven cancer dissemination upon TGFβ stimulation via the RhoGEF GEF-H1 [J].

Biondini, Marco ;

Duclos, Guillaume ;

Meyer-Schaller, Nathalie ;

Silberzan, Pascal ;

Camonis, Jacques ;

Parrini, Maria Carla .

SCIENTIFIC REPORTS, 2015, 5

[9] p53-Responsive MicroRNAs 192 and 215 Are Capable of Inducing Cell Cycle Arrest [J].

Braun, Christian J. ;

Zhang, Xin ;

Savelyeva, Irina ;

Wolff, Sonja ;

Moll, Ute M. ;

Schepeler, Troels ;

Orntoft, Torben F. ;

Andersen, Claus L. ;

Dobbelstein, Matthias .

CANCER RESEARCH, 2008, 68 (24) :10094-10104

[10] Real-time quantification of microRNAs by stem-loop RT-PCR [J].

Chen, CF ;

Ridzon, DA ;

Broomer, AJ ;

Zhou, ZH ;

Lee, DH ;

Nguyen, JT ;

Barbisin, M ;

Xu, NL ;

Mahuvakar, VR ;

Andersen, MR ;

Lao, KQ ;

Livak, KJ ;

Guegler, KJ .

NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9

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