Localisation of low molecular weight sperm antigens during capacitation and acrosome reaction

被引:1
作者
Bathla, H [1 ]
Sidhu, KS [1 ]
机构
[1] Punjab Agr Univ, Dept Zool, Ludhiana 141004, Punjab, India
关键词
hamster; sperm-specific antibodies; sperm surface proteins; capacitation; acrosome reaction; epididymis;
D O I
10.1016/S0378-4320(98)00138-9
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
FITC-labelled sperm-specific antibodies against hamster spermatozoa were utilized as probes in acrosome reaction assays. An indirect immunofluorescence test demonstrated the localisation of two sperm proteins of 19 kDa and 23 kDa on the anterior acrosomal cap region of washed cauda epididymal sperm. These proteins were not detected in reacted acrosome or on immature or immotile sperm. Antisperm agglutinating antibodies specific to these two low molecular weight sperm antigens could be useful probes for evaluating the actosomal status of mammalian spermatozoa. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:55 / 64
页数:10
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