In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes

被引:32
作者
Barani, Mahmood [1 ]
Hajinezhad, Mohammad Reza [2 ]
Sargazi, Saman [3 ]
Rahdar, Abbas [4 ]
Shahraki, Sheida [3 ]
Lohrasbi-Nejad, Azadeh [5 ]
Baino, Francesco [6 ]
机构
[1] Kerman Univ Med Sci, Med Mycol & Bacteriol Res Ctr, Kerman 7616913555, Iran
[2] Univ Zabol, Vet Fac, Basic Vet Sci Dept, Zabol 9861335856, Iran
[3] Res Inst Cellular & Mol Sci Infect Dis, Cellular & Mol Res Ctr, Zahedan 9816743463, Iran
[4] Univ Zabol, Dept Phys, Zabol 9861335856, Iran
[5] Shahid Bahonar Univ Kerman, Dept Agr Biotechnol, Kerman, Iran
[6] Politecn Torino, Inst Mat Phys & Engn, Dept Appl Sci & Technol, Turin, Italy
关键词
ORAL BIOAVAILABILITY; SENSITIVE NANOPARTICLES; REDUCED TOXICITY; DELIVERY; CANCER; NANOTECHNOLOGY; POLYMERS;
D O I
10.1007/s10856-021-06623-6
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In this study, paclitaxel (PTX)-loaded pH-responsive niosomes modified with ergosterol were developed. This new formulation was characterized in terms of size, morphology, encapsulation efficiency (EE), and in vitro release at pH 5.2 and 7.4. The in vitro efficacy of free PTX and niosome/PTX was assessed using MCF7, Hela, and HUVEC cell lines. In order to evaluate the in vivo efficacy of niosomal PTX in rats as compared to free PTX, the animals were intraperitoneally administered with 2.5 mg/kg and 5 mg/kg niosomal PTX for two weeks. Results showed that the pH-responsive niosomes had a nanometric size, spherical morphology, 77% EE, and pH-responsive release in pH 5.2 and 7.4. Compared with free PTX, we found markedly lower IC50s when cancer cells were treated for 48 h with niosomal PTX, which also showed high efficacy against human cancers derived from cervix and breast tumors. Moreover, niosomal PTX induced evident morphological changes in these cell lines. In vivo administration of free PTX at the dose of 2.5 mg/kg significantly increased serum biochemical parameters and liver lipid peroxidation in rats compared to the control rats. The situation was different when niosomal PTX was administered to the rats: the 5 mg/kg dosage of niosomal PTX significantly increased serum biochemical parameters, but the group treated with the 2.5 mg/kg dose of niosomal PTX showed fewer toxic effects than the group treated with free PTX at the same dosage. Overall, our results provide proof of concept for encapsulating PTX in niosomal formulation to enhance its therapeutic efficacy.
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页数:13
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