In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves

被引:47
作者
Li, Yan [1 ]
Sun, Shujuan [1 ]
Guo, Qiongjie [2 ]
Ma, Lin [2 ]
Shi, Changwen [3 ]
Su, Lequn [1 ]
Li, Hongjian [1 ]
机构
[1] Shandong Prov Qianfoshan Hosp, Dept Pharm, Jinan, Shandong Prov, Peoples R China
[2] Shandong Univ, Sch Pharmaceut Sci, Jinan 250100, Shandong Prov, Peoples R China
[3] Shandong Prov Qianfoshan Hosp, Gen Surg Cent Lab, Jinan, Shandong Prov, Peoples R China
关键词
microdilution; spectrophotometric method; non-parametric approaches; colony counting;
D O I
10.1093/jac/dkm493
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: To investigate the in vitro interaction between three azoles (fluconazole, itraconazole and voriconazole) and cyclosporin A against five azole-susceptible (azole-S) and five azole-resistant (azole-R) clinical Candida albicans isolates. Methods: By using a chequerboard technique and time-kill curves, synergistic, indifferent or antagonistic effects when drugs were used in combination were assessed. In the chequerboard assay, the antifungal activity of drug combinations was determined by the microdilution method based on the CLSI M27-A2 guidelines. The effects of the interactions were assessed by two non-parametric approaches (fractional inhibitory concentration index model and Delta E model). In the time-kill assay, a colony counting method was employed against one azole-S strain and one azole-R strain at 0, 6, 12, 24 and 48 h of incubation at 35 degrees C. Results: Good concordance was found between the chequerboard method and time-kill curves. Indifference or synergism was observed for azole-S isolates in interactions of azoles and cyclosporin A, while strong synergism was observed for azole-R isolates in all drug combinations. Conclusions: Cyclosporin A showed potent synergism when combined with the three azoles, especially against azole-R C. albicans strains, and there was good agreement between various methods used in this study.
引用
收藏
页码:577 / 585
页数:9
相关论文
共 28 条
  • [1] [Anonymous], 2002, ANTIMICROB AGENTS CH, DOI DOI 10.1128/AAC.46.3.702.2002
  • [2] [Anonymous], 2002, EMBO J
  • [3] Calcineurin is essential for Candida albicans survival in serum and virulence
    Blankenship, JR
    Wormley, FL
    Boyce, MK
    Schell, WA
    Filler, SG
    Perfect, JR
    Heitman, J
    [J]. EUKARYOTIC CELL, 2003, 2 (03) : 422 - 430
  • [4] FUNGAL-INFECTIONS IN CANCER-PATIENTS - AN INTERNATIONAL AUTOPSY SURVEY
    BODEY, G
    BUELTMANN, B
    DUGUID, W
    GIBBS, D
    HANAK, H
    HOTCHI, M
    MALL, G
    MARTINO, P
    MEUNIER, F
    MILLIKEN, S
    NAOE, S
    OKUDAIRA, M
    SCEVOLA, D
    VANTWOUT, J
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1992, 11 (02) : 99 - 109
  • [5] Synergistic activities of fluconazole and voriconazole with terbinafine against four Candida species determined by checkerboard, time-kill, and Etest methods
    Cantón, E
    Pemán, J
    Gobernado, M
    Viudes, A
    Espinel-Ingroff, A
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (04) : 1593 - 1596
  • [6] Antifungal activities of antineoplastic agents Saccharomyces cerevisiae as a model system to study drug action
    Cardenas, ME
    Cruz, MC
    Del Poeta, M
    Chung, NJ
    Perfect, JR
    Heitman, J
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 1999, 12 (04) : 583 - +
  • [7] In vitro activity of micafungin (FK-463) against Candida spp.:: Microdilution, time-kill, and postantifungal-effect studies
    Ernst, EJ
    Roling, EE
    Petzold, CR
    Keele, DJ
    Klepser, ME
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (12) : 3846 - 3853
  • [8] Assessment of therapeutic response of oropharyngeal and esophageal candidiasis in AIDS with use of a new clinical scoring system: Studies with D0870
    Hood, SV
    Hollis, S
    Percy, M
    Atkinson, G
    Williams, K
    Denning, DW
    [J]. CLINICAL INFECTIOUS DISEASES, 1999, 28 (03) : 587 - 596
  • [9] Influence of test conditions on antifungal time-kill curve results: Proposal for standardized methods
    Klepser, ME
    Ernst, EJ
    Lewis, RE
    Ernst, ME
    Pfaller, MA
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (05) : 1207 - 1212
  • [10] Comparison of Etest, chequerboard dilution and time-kill studies for the detection of synergy or antagonism between antifungal agents tested against Candida species
    Lewis, RE
    Diekema, DJ
    Messer, SA
    Pfaller, MA
    Klepser, ME
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2002, 49 (02) : 345 - 351