SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

被引:43
作者
Chen, Shuangshuang [1 ,2 ,3 ]
Yu, Miao [1 ,2 ]
Li, He [1 ,2 ]
Wang, Ying [1 ,2 ]
Lu, Zhuchou [1 ,2 ]
Zhang, Yunxing [1 ,2 ]
Liu, Mingying [1 ,2 ]
Qiao, Guirong [1 ,2 ]
Wu, Longhua [4 ]
Han, Xiaojiao [1 ,2 ]
Zhuo, Renying [1 ,2 ]
机构
[1] Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China
[2] Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Peoples R China
[3] Jiangsu Acad Agr Sci, Inst Leisure Agr, Nanjing, Peoples R China
[4] Chinese Acad Sci, Inst Soil Sci, Natl Engn Lab Soil Pollut Control & Remediat Tech, Nanjing, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
Sedum alfredii Hance; heat shock transcription factor; cadmium stress; overexpression; ROS-scavenging enzyme; STRESS TOLERANCE; SALT STRESS; TRITICUM-AESTIVUM; HYPERACCUMULATOR; PLANTS; PHYTOREMEDIATION; PHYTOEXTRACTION; ACCUMULATION; CRASSULACEAE; INTEGRATION;
D O I
10.3389/fpls.2020.00142
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup from Sedum alfredii. Designated as SaHsfA4c, the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression of SaHsfA4c in yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcript InSaHsfA4c which contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function of SaHsfA4c under Cd stress was characterized in transgenic Arabidopsis and non-hyperaccumulation ecotype S. alfredii. SaHsfA4c was able to rescue the Cd sensitivity of the Arabidopsis athsfa4c mutant. SaHsfA4c reduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes and Hsps in transgenic lines. The present results suggest that SaHsfA4c increases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression of Hsps.
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页数:14
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