DNA samples from wild animal populations as a byproduct of PIT tagging

被引：0

作者：

Grosser, Stefanie ^{[1
]}

Ratz, Hiltrun

Waters, Jonathan M. ^{[1
]}

机构：

[1] Univ Otago, Allan Wilson Ctr, Dept Zool, Dunedin 9054, New Zealand

来源：

CONSERVATION GENETICS RESOURCES | 2015年 / 7卷 / 03期

关键词：

PIT tag; DNA extraction; Conservation genetics; Penguins; EUDYPTULA-MINOR; PENGUINS; MARKERS;

D O I：

10.1007/s12686-015-0463-1

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We describe a new method for obtaining DNA from needles following their use to insert PIT tags (Passive Integrated Transponders) into wild animals. This approach represents a currently unrealised opportunity, given that PIT tags are a widely-used tool for individual identification. Needles can be frozen and stored long-term and utilised when genetic samples of the study species are required. We demonstrate that DNA can be successfully extracted from PIT tagging needles for amplification of mitochondrial and microsatellite loci in little penguins (Eudyptula minor).

引用

页码：631 / 633

页数：3

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