Complementary analyses of the transcriptome and iTRAQ proteome revealed mechanism of ethylene dependent salt response in bread wheat (Triticum aestivum L.)

被引：26
作者：
Ma, Qian [2 ]
Shi, Changhai [1 ]
Su, Chunxue [2 ]
Liu, Yiguo [1 ]
机构：
[1] Qingdao Agr Univ, Coll Agr, Qingdao 266109, Peoples R China
[2] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
来源：
FOOD CHEMISTRY | 2020年 / 325卷
基金：
中国国家自然科学基金;
关键词：
Ethylene; Salt; Bread wheat; Proteome; Triticum aestivum; Transcriptome; ABIOTIC STRESS; GENE; TOLERANCE; FAMILY; EXPRESSION; RESISTANCE; PLANTS;
D O I：
10.1016/j.foodchem.2020.126866
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In order to clarify the ethylene dependent salt response mechanism in wheat, 2-week-old wheat seedlings of cultivar 'Qingmai 6' treated with water, sodium chloride (NaCl), NaCl and ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and NaCl and ethylene signaling inhibitor 1-methylcyclopropene (1-MCP) were collected and analyzed by transcriptional sequencing and isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. At least 1140 proteins and 73,401 genes were identified, and proteins including ribosomal proteins (RPs), nucleoside diphosphate kinases (CDPKs), transaldolases (TALs), beta-glucosidases (BGLUs), phosphoenlpyruvate carboxylases (PEPCs), superoxide dismutases (SODs), and 6-phosphogluconate dehydrogenases (6-PGDHs) were significantly differently expressed. These genes and proteins revealed that ethylene dependent salt response through RPs activation, chaperones synthesis, the reactive oxygen species (ROS) scavenging, and carbohydrate metabolites pathway. Our results provided transcriptomics and proteomics information with respect to the molecular mechanisms of ethylene regualted salt response.
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页数：10
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共 38 条
[1]   Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity [J].
Abebe, T ;
Guenzi, AC ;
Martin, B ;
Cushman, JC .
PLANT PHYSIOLOGY, 2003, 131 (04) :1748-1755
[2]   Ethylene responsive transcription factor ERF109 retards PCD and improves salt tolerance in plant [J].
Bahieldin, Ahmed ;
Atef, Ahmed ;
Edris, Sherif ;
Gadalla, Nour O. ;
Ali, Hani M. ;
Hassan, Sabah M. ;
Al-Kordy, Magdy A. ;
Ramadan, Ahmed M. ;
Makki, Rania M. ;
Al-Hajar, Abdulrahman S. M. ;
El-Domyati, Fotouh M. .
BMC PLANT BIOLOGY, 2016, 16
[3]   The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists [J].
Baldani, Jose Ivo ;
Reis, Veronica Massena ;
Videira, Sandy Sampaio ;
Boddey, Lucia Helena ;
Divan Baldani, Vera Lucia .
PLANT AND SOIL, 2014, 384 (1-2) :413-431
[4]   Modulation of ethylene responses affects plant salt-stress responses [J].
Cao, Wan-Hong ;
Liu, Jun ;
He, Xin-Jian ;
Mu, Rui-Ling ;
Zhou, Hua-Lin ;
Chen, Shou-Yi ;
Zhang, Jin-Song .
PLANT PHYSIOLOGY, 2007, 143 (02) :707-719
[5]   Expression of the ribosomal proteins S14, S16, L13a and L30 is regulated by cytokinin and abscisic acid -: Implication of the involvement of phytohormones in translational processes [J].
Cherepneva, GN ;
Schmidt, KH ;
Kulaeva, ON ;
Oelmüller, R ;
Kusnetsov, VV .
PLANT SCIENCE, 2003, 165 (05) :925-932
[6]   A role for glutathione transferases functioning as glutathione peroxidases in resistance to multiple herbicides in black-grass [J].
Cummins, I ;
Cole, DJ ;
Edwards, R .
PLANT JOURNAL, 1999, 18 (03) :285-292
[7]   Plant salt-tolerance mechanisms [J].
Deinlein, Ulrich ;
Stephan, Aaron B. ;
Horie, Tomoaki ;
Luo, Wei ;
Xu, Guohua ;
Schroeder, Julian I. .
TRENDS IN PLANT SCIENCE, 2014, 19 (06) :371-379
[8]   Dissecting the proteome dynamics of the salt stress induced changes in the leaf of diploid and autotetraploid Paulownia fortunei [J].
Deng, Minjie ;
Dong, Yanpeng ;
Zhao, Zhenli ;
Li, Yongsheng ;
Fan, Guoqiang .
PLOS ONE, 2017, 12 (07)
[9]   Salicylic acid-related cotton (Gossypium arboreum) ribosomal protein GaRPL18 contributes to resistance to Verticillium dahliae [J].
Gong, Qian ;
Yang, Zhaoen ;
Wang, Xiaoqian ;
Butt, Hamama Islam ;
Chen, Eryong ;
He, Shoupu ;
Zhang, Chaojun ;
Zhang, Xueyan ;
Li, Fuguang .
BMC PLANT BIOLOGY, 2017, 17
[10]   Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves [J].
Jia, Huan ;
Shao, Mingquan ;
He, Yongjun ;
Guan, Rongzhan ;
Chu, Pu ;
Jiang, Haidong .
PLOS ONE, 2015, 10 (12)
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