High-Gossypol Whole Cottonseed Exhibited Mediocre Rumen Degradability and Less Microbial Fermentation Efficiency than Cottonseed Hull and Cottonseed Meal with an In Vitro Gas Production Technique

被引:13
作者
Wang, Weikang [1 ]
Wu, Qichao [1 ]
Li, Wenjuan [1 ]
Wang, Yanlu [1 ]
Zhang, Fan [1 ]
Lv, Liangkang [1 ]
Li, Shengli [1 ]
Yang, Hongjian [1 ]
机构
[1] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
来源
FERMENTATION-BASEL | 2022年 / 8卷 / 03期
关键词
cottonseed by-products; gossypol; in vitro incubation; gas production kinetics; ruminal fermentation characteristics; BACTERIA; TOXICITY;
D O I
10.3390/fermentation8030103
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To explore whether or not the gossypol varied in cottonseed by-products affect rumen degradability and fermentation efficiency, an in vitro cumulative gas production experiment was applied with mixed rumen microorganism to compare rumen fermentation characteristics of whole cottonseed (WCS, n = 3 samples), cottonseed meal (CSM, n = 3 samples), and cottonseed hull (CSH, n = 2 samples). The five-replicate fermentation per sample per incubation time continuously lasted for 0.5, 1.5, 3, 6, 12, 24, 36, and 48 h with an automated gas production recording system. Regardless of distinct nutrient differences, the free gossypol level in these cottonseed by-products ranked: WCS > CSH > CSM. After 48 h of incubation, the in vitro dry matter degradability and ammonia N concentration ranked as: CSM > WCS > CSH. The cumulative gas production and total volatile fatty acid (VFA) levels in the culture fluids ranked: CSM > CSH > WCS, in which the average production rate ranked: CSM > WCS > CSH. Regarding the molar VFA pattern, WCS in comparison with CSH and CSM presented the lowest production of non-glucogenic acids (e.g., acetate) and exhibited the highest fermentation efficiency of energy from carbohydrates to VFAs. There was a significant negative correlation between the gossypol content and cumulative gas and total VFA production, suggesting that the greater gossypol in cottonseed by-products, the more detrimental effect occurred for rumen fermentation. In a brief, WCS exhibited mediocre rumen degradability and less microbial fermentation efficiency than CSH and CSM, depending on their gossypol levels.
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页数:12
相关论文
共 36 条
  • [1] Alexander J, 2008, EFSA J, V6, DOI 10.2903/j.efsa.2008.653
  • [2] AOAC, 1990, Official Methods of Analysis
  • [3] Bagheri N., 2021, ADSA ANN M LOUISV KY
  • [4] BERGMAN EN, 1973, CORNELL VET, V63, P341
  • [5] Brimer L., 2009, EFSA J, V908, P1
  • [6] Chen L., 2015, Gossypol Degradation Strain Coming from Ruminant Rumens and Application Thereof, Patent No. [CN 104328063A, 104328063]
  • [7] Characterization of gossypol-induced sperm abnormalities in bulls
    Chenoweth, PJ
    Chase, CC
    Risco, CA
    Larsen, RE
    [J]. THERIOGENOLOGY, 2000, 53 (05) : 1193 - 1203
  • [8] Effect of urea fertilization on biomass yield, chemical composition, in vitro rumen digestibility and fermentation characteristics of straw of highland barley planted in Tibet
    Cui, J. H.
    Yang, H. J.
    Yu, C. Q.
    Bai, S.
    Wu, T. T.
    Song, S. S.
    Sun, W.
    Shao, X. M.
    Jiang, L. S.
    [J]. JOURNAL OF AGRICULTURAL SCIENCE, 2016, 154 (01) : 151 - 164
  • [9] Effects of soybean oil or various levels of whole cottonseed on intake, digestibility, feeding behavior, and ruminal fermentation characteristics of finishing beef cattle
    de Gouvea, Vinicius Nunes
    Biehl, Marcos Vinicius
    de Castro Ferraz Junior, Marcos Vinicius
    Moreira, Elizangela Mirian
    Faleiro Neto, Jose Alipio
    Westphalen, Mariana Fontana
    Oliveira, Gabriela Bagio
    Ferreira, Evandro Maia
    Polizel, Daniel Montanher
    Pires, Alexandre Vaz
    [J]. LIVESTOCK SCIENCE, 2021, 244
  • [10] Reversibility of the reproductive toxicity of gossypol in peripubertal bulls
    Hassan, ME
    Smith, GW
    Ott, RS
    Faulkner, DB
    Firkins, LD
    Ehrhart, EJ
    Schaeffer, DJ
    [J]. THERIOGENOLOGY, 2004, 61 (06) : 1171 - 1179