Curcumin Affects Gastric Cancer Cell Migration, Invasion and Cytoskeletal Remodeling Through Gli1-β-Catenin

被引：37
作者：
Zhang, Xiao [1 ]
Zhang, Chenli [1 ]
Ren, Zhiheng [1 ]
Zhang, Fangfang [1 ]
Xu, Jinyu [1 ]
Zhang, Xu [1 ]
Zheng, Haixue [2 ]
机构：
[1] Lanzhou Univ, Sch Basic Med, Dept Pathol, 222 Tianshui Rd, Lanzhou 730000, Gansu, Peoples R China
[2] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Natl Foot & Mouth Dis Reference Lab, 1 Yanchang Rd, Lanzhou 730000, Gansu, Peoples R China
来源：
CANCER MANAGEMENT AND RESEARCH | 2020年 / 12卷
关键词：
curcumin; Gli1; beta-catenin; migration; invasion; cytoskeleton; HEDGEHOG SIGNALING PATHWAY; SONIC HEDGEHOG; BETA-CATENIN; HEPATOCELLULAR-CARCINOMA; WNT/BETA-CATENIN; MESENCHYMAL TRANSITION; ACTIVATION; EXPRESSION; APOPTOSIS; PROLIFERATION;
D O I：
10.2147/CMAR.S244384
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Purpose: The function of curcumin on the gastric cancer cell line, SGC-7901 is unknown. The present study aimed to observe the effects of curcumin on gastric cancer cells through the Shh and Wnt signaling pathways. Methods: SGC-7901 cells were transfected with si-Gli1 and si-beta-catenin siRNA, then cells were stimulated with curcumin and its effects on cell migration, invasion, cytoskeleton remodeling, EMT, apoptosis and cell cycle were investigated by transwell assays, immunofluorescence and flow cytometry assays. The interaction between Glil and beta-catenin was observed by co-immunoprecipitation. Results: We show that curcumin suppressed the expression of Shh, Gli1 and Foxm1 in the Shh signaling pathway, and the expression of beta-catenin in the Wnt signaling pathway in SGC-7901 cells, both in mRNA and protein. As a result, cellular migration, invasion and cytoskeletal remodeling ability decreased. Our results revealed that when stimulated with curcumin, cells showed decreased cellular migration and invasion, while enhanced apoptosis. In addition, curcumin induced cytoskeletal remodeling and S phase cell cycle arrest. The inhibition of Shh and Wnt signaling pathway and the addition of curcumin also inhibited the epithelial-mesenchymal transition process. Furthermore, a physical interaction was observed between Glil of the Shh signaling and beta-catenin of the Wnt signaling in these cells, but curcumin inhibited the interaction of these two proteins. Conclusion: The present study indicated that curcumin plays an anti-tumor role through Gli1-beta-catenin pathway in gastric cancer SGC-7901 cells.
引用
收藏
页码：3795 / 3806
页数：12
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