Adequate Disinfection of a Split-Septum Needleless Intravascular Connector with a 5-Second Alcohol Scrub

被引：30

作者：

Rupp, Mark E. ^{[1
,2
]}

Yu, Stephanie ^{[1
]}

Huerta, Tomas ^{[1
]}

Cavalieri, R. Jennifer ^{[1
]}

Alter, Roxanne ^{[3
]}

Fey, Paul D. ^{[3
]}

Van Schooneveld, Trevor ^{[1
,2
]}

Anderson, James R. ^{[4
]}

机构：

[1] Univ Nebraska, Med Ctr, Dept Internal Med, Omaha, NE USA

[2] Nebraska Med Ctr, Dept Infect Control & Epidemiol, Omaha, NE USA

[3] Univ Nebraska, Med Ctr, Dept Pathol & Microbiol, Omaha, NE USA

[4] Univ Nebraska, Med Ctr, Coll Publ Hlth, Dept Biostat, Omaha, NE USA

来源：

INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY | 2012年 / 33卷 / 07期

关键词：

BLOOD-STREAM INFECTIONS; CATHETER; RECOMMENDATIONS; CONTAMINATION; VALVE; RISK;

D O I：

10.1086/666337

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

OBJECTIVE. Define optimum vascular catheter connector valve disinfection practices under laboratory and clinical conditions. DESIGN. Prospective observational clinical survey and laboratory assessment of disinfection procedures. SETTING. All adult inpatients at an academic healthcare center. METHODS. In the clinical setting, contamination of needleless connectors was assessed in 6 weekly prevalence surveys in which the connector valves from central venous catheters (CVCs) in situ were cultured by pressing the connector diaphragm to an agar plate. Before culture, valves were disinfected by scrubbing the diaphragm with a 70% isopropyl alcohol pledget for 0, 5, 10, 15, or 30 seconds. In the laboratory, the diaphragms on 150 unused sterile connector valves were inoculated with 10(3), 10(5), or 10(8) colony-forming units of Staphylococcus epidermidis and allowed to dry. After disinfection of the diaphragms by scrubbing with a 70% isopropyl alcohol pledget for 0, 5, 10, 15, or 30 seconds, the valves were sampled by pressing the diaphragm to an agar plate. RESULTS. In the clinical setting, 363 connector valves from patients with CVCs were sampled, and 66.7% of nondisinfected valves revealed bacterial contamination. After 5-second disinfection with an alcohol pledget, only 1 (1.4%) of 71 yielded microbial growth (P < .005). In the laboratory, at the 10(3) and 10(5) inoculum, all connector valves yielded sterile cultures when scrubbed for 5 or more seconds (P < .001). At the 10(8) inoculum, 2 (20%) of 10 connector valves yielded minimal growth of S. epidermidis. CONCLUSIONS. A 5-second scrub with a 70% isopropyl alcohol pledget yields adequate disinfection of a split-septum intravascular catheter connector valve under clinical and laboratory conditions. Infect Control Hosp Epidemiol 2012;33(7):661-665

引用

页码：661 / 665

页数：5