Detection of the genes for Staphylococcus aureus enterotoxin by PCR

The aim of this study was to determine the presence of genes for staphylococcal enterotoxins in group of 32 isolates of Staphylococcus aureus from 75 samples (sheep, cow and goat raw milk) collected from sub-clinically and clinically sick sheep, goats and cows raised in different agricultural farms in the Eastern region of Slovakia and also from 12 samples of sheep cheeses. Fifteen out of 32 isolates of S. aureus carried gene for enterotoxin C (SEC): 12 from sheep raw milk and one from each of goat and cow raw milk and sheep cheese, too. Fifteen out of 32 isolates carried gene for enterotoxin D (SED): 12 from sheep raw milk, two from cow raw milk and one from sheep cheese. Two out of 32 isolates carried genes for enterotoxin A (SEA) and B (SEB): one positive isolate for SEA from sheep raw milk and the other SEB from sheep cheese.