Gene expression of pepsinogen during the larval development of red porgy (Pagrus pagrus)

被引:60
作者
Darias, MJ
Murray, HM
Martínez-Rodríguez, G
Cárdenas, S
Yúfera, M
机构
[1] CSIC, Inst Ciencias Marinas Andalucia, E-11510 Puerto Real, Spain
[2] Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada
[3] CIFPA Toruno, E-11500 Pto Sta Maria, Spain
关键词
Pagrus pagrus; pepsinogen; gastric glands; stomach acidification;
D O I
10.1016/j.aquaculture.2005.04.044
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Ontogeny of stomach functionality during the larval development of the red porgy was studied from morphological, physiological and molecular perspectives. The aim of this study was to advance our understanding of the sequence of events occurring during the transition from alkaline to acid digestion in sparids. Gastric gland histological development was examined using haematoxylin-eosin staining. Pepsinogen mRNA was localized using in situ hybridization on 5-7 mu m sections of paraffin embedded samples. Gastric pH in the lumen of the stomach was measured using a pH microelectrode. The first signs of gastric glands were apparent by 19 days alter hatching (dah) and were fully morphologically developed on 26 dab approximately. Pepsinogen expression was evidenced in the gastric glands from 30 dab onwards. The decrease in gastric pH was detected in fed larvae above 35 dah. (C) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码:245 / 252
页数:8
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