Touch DNA sampling with SceneSafe Fast™ minitapes

被引:19
作者
Stoop, Britta [1 ]
Defaux, Priscille Merciani [2 ]
Utz, Silvia [1 ]
Zieger, Martin [1 ]
机构
[1] Univ Bern, Inst Forens Med, Forens Mol Biol Dpt, Sulgenauweg 40, CH-3007 Bern, Switzerland
[2] Bern Cantonal Police, Nordring 30, CH-3001 Bern, Switzerland
关键词
Trace DNA; Clothing; Sampling; Tape; DNA extraction; FORENSIC ADHESIVE TAPES; COLLECTION; EXTRACTION;
D O I
10.1016/j.legalmed.2017.10.006
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
To achieve optimal results in the forensic analysis of trace DNA, choosing the right collection technique is crucial. Three common approaches are currently well-established for DNA retrieval from items of clothing, notably cutting, swabbing and tape-lifting. The latter two are non-destructive and therefore preferable on items of value. Even though the most recently established technique of DNA retrieval by adhesive tapes is widely used since quite some years now, little information has been published so far on how well it performs compared to other methods. Even more important, when it comes to choosing the right DNA extraction method for forensic lifting-tapes, the available information one can rely on as a forensic geneticist is quite scarce. In our study we compared the two widely used, commercially available and automation suitable magnetic bead-based extraction methods "iPrep Forensic Kit" and "PrepFiler Express BTA (TM) Kit" to conventional organic solvent extraction. The results demonstrate that DNA extraction from standardized saliva samples applied to SceneSafe Fast (TM) minitapes is most efficient with phenol chloroform. We also provide evidence that SceneSafe Fast (TM) minitapes perform better than wet cotton swabs in the sampling of touch DNA from cotton fabric. Applying the tape only once in every spot on the tissue is thereby sufficient for a considerably better collection performance of the tapes compared to swabbing.
引用
收藏
页码:68 / 71
页数:4
相关论文
共 12 条
[1]   The Use of Adhesive Tape for Recovery of DNA from Crime Scene Items [J].
Barash, Mark ;
Reshef, Ayeleth ;
Brauner, Paul .
JOURNAL OF FORENSIC SCIENCES, 2010, 55 (04) :1058-1064
[2]   High-throughput DNA extraction of forensic adhesive tapes [J].
Forsberg, Christina ;
Jansson, Linda ;
Ansell, Ricky ;
Hedman, Johannes .
FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2016, 24 :158-163
[3]   Trace DNA collection-Performance of minitape and three different swabs [J].
Hansson, Oskar ;
Finnebraaten, Marianne ;
Heitmann, Ingebjorg Knutsen ;
Ramse, Merete ;
Bouzga, Mariam .
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2009, 2 (01) :189-190
[4]   A comparison of four methods for PCR inhibitor removal [J].
Hu, Qingqing ;
Liu, Yuxuan ;
Yi, Shaohua ;
Huang, Daixin .
FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2015, 16 :94-97
[5]   DNA extraction of forensic adhesive tapes-A comparison of two different methods [J].
Joel, J. ;
Glanzmann, B. ;
Germann, U. ;
Cossu, C. .
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2015, 5 :E579-E581
[6]   Everything clean? Transfer of DNA traces between textiles in the washtub [J].
Kamphausen, Thomas ;
Fandel, Sabine Birgit ;
Gutmann, Jochen Stefan ;
Bajanowski, Thomas ;
Poetsch, Micaela .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2015, 129 (04) :709-714
[7]  
Malik N., 2008, 19 INT S HUM ID HOLL
[8]   Optimisation of cellular DNA recovery from tape-lifts [J].
May, R. ;
Thomson, J. .
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2009, 2 (01) :191-192
[9]   DNA transfer during laundering may yield complete genetic profiles [J].
Noel, Sarah ;
Lagace, Karine ;
Rogic, Anita ;
Granger, Dominic ;
Bourgoin, Sarah ;
Jolicoeur, Christine ;
Seguin, Diane .
FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2016, 23 :240-247
[10]   Nondestructive Biological Evidence Collection with Alternative Swabs and Adhesive Lifters [J].
Plaza, Dane T. ;
Mealy, Jamia L. ;
Lane, J. Nicholas ;
Parsons, M. Neal ;
Bathrick, Abigail S. ;
Slack, Donia P. .
JOURNAL OF FORENSIC SCIENCES, 2016, 61 (02) :485-488