Chiral Nano-Liquid Chromatography and Dispersive Liquid-Liquid Microextraction Applied to the Analysis of Antifungal Drugs in Milk

被引:10
作者
Dal Bosco, Chiara [1 ]
Bonoli, Flavia [1 ]
Gentili, Alessandra [1 ]
Fanali, Chiara [2 ]
D'Orazio, Giovanni [3 ]
机构
[1] Sapienza Univ Rome, Dept Chem, I-00185 Rome, Italy
[2] Univ Campus Biomed Roma, Dept Sci & Technol Humans & Environm, Unit Food Sci & Nutr, I-00128 Rome, Italy
[3] CNR, Ist & Sistemi Biol ISB, I-00015 Rome, Italy
来源
MOLECULES | 2021年 / 26卷 / 23期
关键词
antifungal drugs; chiral separation; nano-LC; dispersive liquid-liquid microextraction; milk; SIMULTANEOUS ENANTIOSELECTIVE DETERMINATION; SOLID-PHASE EXTRACTION; TRIAZOLE FUNGICIDES; ENANTIOMERIC SEPARATION; POLYMER MONOLITHS; MASS-SPECTROMETRY; ESTROGENIC COMPOUNDS; AZOLE ANTIFUNGALS; STATIONARY PHASES; ANIMAL HEALTH;
D O I
10.3390/molecules26237094
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel chromatographic application in chiral separation by using the nano-LC technique is here reported. The chiral recognition of 12 antifungal drugs was obtained through a 75 mu m I.D. fused-silica capillary, which was packed with a CSP-cellulose 3,5-dichlorophenylcarbamate (CDCPC), by means of a lab-made slurry packing procedure. The mobile phase composition and the experimental conditions were optimized in order to find the optimum chiral separation for some selected racemic mixtures of imidazole and triazole derivatives. Some important parameters, such as retention faction, enantioresolution, peak efficiency, and peak shape, were investigated as a function of the mobile phase (pH, water content, type and concentration of both the buffer and the organic modifier, and solvent dilution composition). Within one run lasting 25 min, at a flow rate of approximately 400 nL min(-1), eight couples of enantiomers were baseline-resolved and four of them were separated in less than 25 min. The method was then applied to milk samples, which were pretreated using a classical dispersive liquid-liquid microextraction technique preceded by protein precipitation. Finally, the DLLME-nano-LC-UV method was validated in a matrix following the main FDA guidelines for bioanalytical methods.
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页数:16
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共 41 条
[1]   A chiral enantioseparation generic strategy for anti-Alzheimer and antifungal drugs by short end injection capillary electrophoresis using an experimental design approach [J].
Abdel-Megied, Ahmed M. ;
Hanafi, Rasha S. ;
Aboul-Enein, Hassan Y. .
CHIRALITY, 2018, 30 (02) :165-176
[2]   Single-walled carbon nanotube-based polymer monoliths for the enantioselective nano-liquid chromatographic separation of racemic pharmaceuticals [J].
Ahmed, Marwa ;
Yajadda, Mir Massoud Aghili ;
Han, Zhao Jun ;
Su, Dawei ;
Wang, Guoxiu ;
Ostrikov, Kostya ;
Ghanem, Ashraf .
JOURNAL OF CHROMATOGRAPHY A, 2014, 1360 :100-109
[3]   Chiral β-cyclodextrin functionalized polymer monolith for the direct enantioselective reversed phase nano liquid chromatographic separation of racemic pharmaceuticals [J].
Ahmed, Marwa ;
Ghanem, Ashraf .
JOURNAL OF CHROMATOGRAPHY A, 2014, 1345 :115-127
[4]   Residue analysis of glucocorticoids in bovine milk by liquid chromatography-tandem mass spectrometry [J].
Caretti, Fulvia ;
Gentili, Alessandra ;
Ambrosi, Annalisa ;
Rocca, Lucia Mainero ;
Delfini, Maurizio ;
Di Cocco, Maria Enrica ;
D'Ascenzo, Giuseppe .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (06) :2477-2490
[5]   CHLOROMETHYLPHENYLCARBAMATE DERIVATIVES OF CELLULOSE AS CHIRAL STATIONARY PHASES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
CHANKVETADZE, B ;
YASHIMA, E ;
OKAMOTO, Y .
JOURNAL OF CHROMATOGRAPHY A, 1994, 670 (1-2) :39-49
[6]   DIMETHYLPHENYLCARBAMATES, DICHLOROPHENYLCARBAMATES AND CHLOROMETHYLPHENYLCARBAMATES OF AMYLOSE AS CHIRAL STATIONARY PHASES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
CHANKVETADZE, B ;
YASHIMA, E ;
OKAMOTO, Y .
JOURNAL OF CHROMATOGRAPHY A, 1995, 694 (01) :101-109
[7]   Nano-liquid chromatography for enantiomers separation of baclofen by using vancomycin silica stationary phase [J].
D'Orazio, Giovanni ;
Fanali, Chiara ;
Gentili, Alessandra ;
Tagliaro, Franco ;
Fanali, Salvatore .
JOURNAL OF CHROMATOGRAPHY A, 2019, 1605
[8]   Enantiomeric separation of some chiral analytes using amylose 3,5-dimethylphenylcarbamate covalently immobilized on silica by nano-liquid chromatography and capillary electrochromatography [J].
D'Orazio, Giovanni ;
Fanali, Chiara ;
Karchkhadze, Marina ;
Chankvetadze, Bezhan ;
Fanali, Salvatore .
JOURNAL OF CHROMATOGRAPHY A, 2017, 1520 :127-134
[9]   Determination of estrogenic compounds in milk and yogurt samples by hollow-fibre liquid-phase microextraction-gas chromatography-triple quadrupole mass spectrometry [J].
D'Orazio, Giovanni ;
Hernandez-Borges, Javier ;
Vicente Herrera-Herrera, Antonio ;
Fanali, Salvatore ;
Angel Rodriguez-Delgado, Miguel .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (26) :7447-7459
[10]   Evaluation of the combination of a dispersive liquid-liquid microextraction method with micellar electrokinetic chromatography coupled to mass spectrometry for the determination of estrogenic compounds in milk and yogurt [J].
D'Orazio, Giovanni ;
Asensio-Ramos, Maria ;
Hernandez-Borges, Javier ;
Angel Rodriguez-Delgado, Miguel ;
Fanali, Salvatore .
ELECTROPHORESIS, 2015, 36 (04) :615-625