Performance evaluation and phylogenetic characterization of anaerobic fluidized bed reactors using ground tire and pet as support materials for biohydrogen production

被引：40

作者：

Barros, Aruana Rocha ^{[1
]}

Tallarico Adorno, Maria Angela ^{[2
]}

Sakamoto, Isabel Kimiko ^{[2
]}

Maintinguer, Sandra Imaculada ^{[2
]}

Amancio Varesche, Maria Bernadete ^{[2
]}

Silva, Edson Luiz ^{[1
]}

机构：

[1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP, Brazil

[2] Univ Sao Paulo, Dept Hydraul & Sanitat, BR-13566590 Sao Carlos, SP, Brazil

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

Biohydrogen production; Anaerobic fluidized bed reactor; Ground tire; PET; 16S rRNA; FERMENTATIVE HYDROGEN-PRODUCTION; MICROBIAL COMMUNITY; BACTERIAL COMMUNITIES; GLUCOSE; DNA; PH;

D O I：

10.1016/j.biortech.2010.12.014

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This study evaluated two different support materials (ground tire and polyethylene terephthalate [PET]) for biohydrogen production in an anaerobic fluidized bed reactor (AFBR) treating synthetic wastewater containing glucose (4000 mg L-1). The AFBR, which contained either ground tire (R1) or PET (R2) as support materials, were inoculated with thermally pretreated anaerobic sludge and operated at a temperature of 30 degrees C. The AFBR were operated with a range of hydraulic retention times (HRT) between 1 and 8 h. The reactor R1 operating with a HRT of 2 h showed better performance than reactor R2, reaching a maximum hydrogen yield of 2.25 mol H-2 mol(-1) glucose with 1.3 mg of biomass (as the total volatile solids) attached to each gram of ground tire. Subsequent 16S rRNA gene sequencing and phylogenetic analysis of particle samples revealed that reactor R1 favored the presence of hydrogen-producing bacteria such as Clostridium, Bacillus, and Enterobacter. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：3840 / 3847

页数：8

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