Somatic Embryogenesis in Cyclamen with Two Explants and Combinations of Plant Growth Regulators

被引：0
|
作者：
Jalali, Neda [1 ]
Naderi, Roohangiz [1 ]
Babalar, Mesbah [1 ]
Mirmasoumi, Masoud [2 ]
机构：
[1] Univ Tehran, Dept Hort Sci, Fac Agr, Karaj 3158777871, Iran
[2] Univ Tehran, Dept Plant Sci, Coll Sci, Fac Biol, Tehran, Iran
来源：
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY | 2010年 / 51卷 / 05期
关键词：
2,4-D; calus; Cyclamen persicum Mill; kinetin; plant regeneration; somatic embryo; PERSICUM; REGENERATION; IMPROVEMENT;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Cyclamen persicum Mill. (Myrsinaceae) is one of the most important winter pot flowers in the world. It is commercially propagated via F-1-hybrid seeds. Somatic embryogenesis is an efficient method of micropropagation and biotechnological tools for genetic improvement in cyclamen. The effect of initial explants type (leaf and petiole) on callus growth and somatic embryo were studied. Somatic embryos were cultured on modified Murashige and Skoog (MS) medium. Also embryogenic callus was induced on several media containing 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (Kin). The greatest amount of callus was observed in two explants on media containing 1 mg.L-1 2,4-D + 0.5 mg.L-1 Kin. The optimal responses of somatic emberyogenesis were recorded on media enriched with 4 mg.L-1 2,4-D, 0.1 mg.L-1 Kin and leaf explants. These results were obtained from adult plants.
引用
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页码：445 / 448
页数：4
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