EPR-Based Approach for the Localization of Paramagnetic Metal Ions in Biomolecules

被引：58

作者：

Abdullin, Dinar ^{[1
]}

Florin, Nicole ^{[1
]}

Hagelueken, Gregor ^{[1
]}

Schiemann, Olav ^{[1
]}

机构：

[1] Univ Bonn, Inst Phys & Theoret Chem, D-53115 Bonn, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 06期

关键词：

binding sites; copper; EPR spectroscopy; spin labels; triangulation; SPIN-DENSITY DISTRIBUTION; PROTEIN-STRUCTURE; BINDING; RESONANCE; SELECTION; AZURIN; SITES; ELDOR; NMR;

D O I：

10.1002/anie.201410396

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three-dimensional fold of such biomolecules is therefore an important goal in understanding structure-function relationships. A trilateration approach for the localization of metal ions by means of long-range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu2+ center of azurin, and factors affecting the precision of the method are discussed.

引用

页码：1827 / 1831

页数：5

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