Analyses of the cis-Regulatory Regions Responsible for the Transcriptional Activation of the N Resistance Gene by Tobacco mosaic virus

被引：10

作者：

Kobayashi, Michie ^{[1
]}

Ishihama, Nobuaki ^{[2
]}

Yoshioka, Hirofumi ^{[2
]}

Takabatake, Reona ^{[3
]}

Tsuda, Shinya ^{[4
]}

Seo, Shigemi ^{[1
]}

Ohashi, Yuko ^{[1
]}

Mitsuhara, Ichiro ^{[1
]}

机构：

[1] Natl Inst Agrobiol Sci, Plant Microbe Interact Res Unit, Tsukuba, Ibaraki 3058602, Japan

[2] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

[3] Natl Agr & Food Res Org, Natl Food Res Inst, GMO Analyt Evaluat Lab, Tsukuba, Ibaraki 3058642, Japan

[4] Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan

来源：

JOURNAL OF PHYTOPATHOLOGY | 2010年 / 158卷 / 11-12期

关键词：

N resistance gene; Tobacco mosaic virus; transcription; EXPRESSION; VECTOR;

D O I：

10.1111/j.1439-0434.2010.01686.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Although the tobacco N gene, which confers resistance to Tobacco mosaic virus (TMV), is transcriptionally induced by infection with TMV, the regulatory mechanism has not previously been elucidated. We found that a 5'-flanking region of the N gene exhibits TMV-inducible promoter activity and that the region from -290 to -271 includes a cis-acting element in response to infection. The activity required N protein and signal components of N-mediated resistance. These results suggest that transcription of the N gene is positively regulated by N-mediated resistance signalling.

引用

页码：826 / 828

页数：3

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