DNA methylation status of P2 promoter of IGF2 gene in growth restricted neonates

被引：1

作者：

Sibin, M. K. ^{[1
]}

Hothi, Onkar ^{[1
]}

Misra, Pratibha ^{[1
]}

Manoj, P. ^{[1
]}

Chakravarty, Rony ^{[1
,2
]}

Shaw, Subhash Chandra ^{[3
]}

Kanitkar, Madhuri ^{[4
]}

机构：

[1] Armed Forces Med Coll, Dept Biochem, Pune 411040, Maharashtra, India

[2] Armed Forces Med Coll, Dept Obstet & Gynaecol, Pune 411040, Maharashtra, India

[3] Armed Forces Med Coll, Dept Paediat, Pune 411040, Maharashtra, India

[4] DCIDS Med, New Delhi, India

来源：

GENE REPORTS | 2021年 / 23卷

关键词：

IUGR; IGF-2; P2 promoter methylation; SGA; MS-PCR; FOR-GESTATIONAL-AGE; FETAL-GROWTH; INTRAUTERINE GROWTH; BIRTH-WEIGHT; CORD BLOOD; ASSOCIATION; EXPRESSION; PLACENTAS; MODULATOR; INFANTS;

D O I：

10.1016/j.genrep.2021.101153

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Objective: Small for gestational age (SGA) is associated with epigenetic changes in DNA methylation status of various gene promoters as studied in cord blood of neonates and placenta samples of their mothers. Changes in methylation status of IGF-2 gene promoter may down regulate its expression and thereby control the weight and growth of babies. Objective of the current study was to determine the P2 promoter methylation status of IGF-2 gene in SGA neonates. Study design: Twenty-eight growth restricted and 29 appropriate for gestational age (AGA) neonates were recruited in this study and umbilical cord blood samples were collected at birth. DNA samples were isolated and subjected to bisulfite modification and using specific primers methylation specific (MS) PCR was performed. The status of methylation in P2 promoter of IGF-2 was recorded after agarose gel electrophoresis. Results: Hypo methylation of P2 promoter of IGF-2 promoter was found in 64.3% of cord blood samples of growth restricted neonates as compared to 14% of AGA (p < 0.001). However, there was no correlation between methylation status and other risk factors of SGA. Conclusion: IGF-2 P2 promoter hypo methylation is found in majority of cord blood samples of Indian growth restricted neonates.

引用

页数：5

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