(S)-(-)-N-[2-(3-hydroxy-2-oxo-2,3-dihydro-1H-indol-3-yl)-ethyl]-acetamide Inhibits Neuroglioma Cell Growth Through Inducing Apoptosis

Neuroglioma is an aggressive type of cancer in the central nervous system without effective treatments. (S)-(-)-N-[2-(3-hydroxy-2-oxo-2,3-dihydro-1H-indol-3-yl)-ethyl]-acetamide (SNA) is a new compound isolated from Selaginella pulvinata plant with potent inhibitory activity against melanoma cell proliferation through increasing apoptosis. This study further investigated effects of SNA against neuroglioma and explored the underlying mechanisms. Neuroglioma H4 cells were treated with various SNA concentrations and MTT assay was used to detect the SNA effects on cell proliferation. Wound healing and cell invasion assays were used to measure the cell migration, Annexin V/PI staining was used to detect apoptosis. Western blot was used to detect the expression of proteins. SNA significantly inhibited the proliferation of H4 cells and suppressed the cell migration. Moreover, SNA increased cell cycle arrest and induced the apoptosis. Our data demonstrated that SNA could be a potential anti-tumor drug against neuroglioma.