Drosophila sex-lethal inhibits the stable association of the 40S ribosomal subunit with msl-2 mRNA

被引：56
作者：
Gebauer, F [1 ]
Grskovic, M [1 ]
Hentze, MW [1 ]
机构：
[1] European Mol Biol Lab, Gene Express Programme, D-69117 Heidelberg, Germany
来源：
MOLECULAR CELL | 2003年 / 11卷 / 05期
关键词：
D O I：
10.1016/S1097-2765(03)00176-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The inhibition of male-specific lethal-2 (msl-2) mRNA translation in female files is essential for X chromosome dosage compensation in Drosophila melanogaster. Translational repression of mst-2 requires sex-lethal (SXL) binding to uridine-rich sequences in both the 5' and 3' untranslated regions (UTRs) of the message. We delineate the msl-2 mRNA sequence elements that are important for regulation by SXL and identify functionally critical sequences adjacent to regulatory SXL binding sites. We demonstrate that SXL inhibits translation initiation and prevents the stable association of the 40S ribosomal subunit with the mRNA in a manner that does not require the presence of a cap structure at the 5' end of the mRNA. These results elucidate a critical regulatory step for dosage compensation in Drosophila melanogaster.
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页码：1397 / 1404
页数：8
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