Impaired water reabsorption in mice deficient in the type VI adenylyl cyclase (AC6)

被引：33

作者：

Chien, Chen-Li ^{[1
,2
]}

Wu, Yu-Shuo ^{[1
,2
]}

Lai, Hsing-Lin ^{[1
]}

Chen, Yen-Hui ^{[1
]}

Jiang, Si-Tse ^{[3
]}

Shih, Chia-Ming ^{[1
]}

Lin, Sui-Shan ^{[1
]}

Chang, Chen ^{[1
]}

Chern, Yijuang ^{[1
,2
]}

机构：

[1] Acad Sinica, Inst Biomed Sci, Taipei 115, Taiwan

[2] Natl Def Med Ctr, Inst Life Sci, Taipei, Taiwan

[3] Natl Appl Res Labs, Natl Lab Anim Ctr, Tainan, Taiwan

来源：

FEBS LETTERS | 2010年 / 584卷 / 13期

关键词：

Adenylyl cyclase; cAMP; Water reabsorption; Renal tubule; Collecting duct; Kidney; KIDNEY COLLECTING DUCT; CENTRAL-NERVOUS-SYSTEM; CAMP CONTENT; EXPRESSION; CELLS; LOCALIZATION; MECHANISMS; RECEPTORS; MODELS; DNA;

D O I：

10.1016/j.febslet.2010.05.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adenylyl cyclase (AC) type VI (AC6) is a calcium-inhibitable enzyme which produces cAMP upon stimulation. Herein, we characterized the specific role of AC6 in the kidneys using two AC6-knockout mouse lines. Immunohistochemical staining revealed that AC6 exists in the tubular parts of the nephron and collecting duct. Activities of AC evoked by forskolin or a selective agonist of the V2 vasopressin receptor were lower in the kidneys of AC6-null mice compared to those of wildtype mice. Results of a metabolic cage assay and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) showed for the first time that AC6 plays a critical role in regulating water homeostasis. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：2883 / 2890

页数：8

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