Diagnosis of Glanzmann thrombasthenia by whole blood impedance analyzer (MEA) vs. light transmission aggregometry

BackgroundGlanzmann thrombasthenia (GT) is a rare inherited platelet disorder that is characterized by spontaneous or postprocedural bleeding. The diagnosis of GT depends on identifying the dysfunction of the platelets. AimThe aim of this study was to compare a whole blood impedance Multiplate analyzer (MEA) with the standard method, light transmission aggregometry (LTA) in diagnosis of GT. MethodsFifteen patients with GT were assessed on MEA and LTA using arachidonic acid (ASPI: 15mm), (TRAP: 1mm), collagen (100g/mL), ADP (0.2mm), and ristocetin (Risto: 10mg/mL). Whole blood samples were collected in sodium citrate and hirudin vacuum, blood collection tubes and tested within 4h. Platelet-rich plasma was used for LTA using platelet agonists (ristocetin 1.5mg/mL) (arachidonic acid 0.5mg/mL) (ADP 2.5mg/mL) and (collagen 1mg/mL). ResultsThe platelet count and PFA-100 results were (average and SD) 31993x10(9)L and 252 +/- 34s, respectively. Flow cytometry analysis showed that all samples are positive for CD42a and CD42b, whereas 9/15 samples were negative for CD61 and CD41. The other six patients had either partial or full expression of CD61/CD41. Aggregation analysis using both methods showed that all samples had no aggregation response to any of the agonists used apart from six samples which, using only the MEA, showed minimal aggregation in response to collagen (average=14.3 +/- 7g, which may suggest ability to detect qualitative abnormality of GPIIb/IIIa). ConclusionThese results suggest that the MEA is sensitive for the detection of Glanzmann thrombasthenia. Furthermore, MEA may also be able to differentiate between the subtypes of Glanzmann thrombasthenia.