Hydrogen Peroxide Biosensor Based on Cellulose Diacetate-Ionic Liquid Film Immobilizing Myoglobin

The myoglobin (Mb) was immobilized on the surface of glassy carbon electrode (GCE) by using composite of cellulose diacetate (CDA) and ionic liquid 1-butyl-3-methylimiclazolium tetrafluoroborate ([bmim]BF(4)). Spectroscopic analysis of the CDA-[bmim]BF(4)-Mb film showed that the Mb could retain its natural structure and bioactivity. The conductivity of the modified electrode had been greatly improved due to the doping of ionic liquid, which facilitated the electronic transfer of the CDA-[bmim]BF(4)-Mb membrane. Further experiment demonstrated that the Mb modified electrode exhibited an electrocatalytic activity for the reduction of H(2)O(2). The catalytic peak current was linear to H(2)O(2) concentration in the range of 5.0-100 mu mol/L with a detection limit of 2.0 mu mol/L. The apparent Michaelis-Menten constant (K(m)) was estimated to be 1.758X10(-4) mol/L.