Role of cellular phosphatase cdc25C in herpes simplex virus 1 replication

被引:5
作者
Smith-Donald, Benjamin A. [1 ]
Durand, Lizette O. [1 ]
Roizman, Bernard [1 ]
机构
[1] Univ Chicago, Marjorie B Kovler Viral Oncol Labs, Chicago, IL 60637 USA
关键词
D O I
10.1128/JVI.02021-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Earlier studies have shown that in herpes simplex virus 1-infected cells, ICP22 upregulates the accumulation of a subset of gamma(2) proteins exemplified by the products of the U(L)38, U(L)41, and U(s)11 genes. The ICP22-dependent process involves degradation of cyclins A and B1, the stabilization and activation of cdc2, physical interaction of activated cdc2 with the U(L)42 DNA synthesis processivity factor, and recruitment and phosphorylation of topoisomerase Hot by the cdc2/U(L)42 complex. Activation of cdc2, the first step in the process, is a key function of the mitotic phosphatase cdc25C. To define the role of cdc25C, we probed some features of the ICP22-dependent pathway of upregulation of gamma(2) genes in cdc25C(-/-) cells and in cdc25C(-/-) cells derived from sibling mice. We report that cyclin B1 turned over in cdc25C(+/+) or cdc25C(-/-) cells at the same rate, that cdc2 increased in amount, and that U(s)11 and U(L)38 proteins and infectious virus accumulated in smaller amounts than in wild-type infected cells. The reduction in U(L)38 protein accumulation and virus was greater in cdc25C(-/-) cells infected with virus lacking ICP22 than in cells infected with wild-type virus. We conclude that cdc25C phosphatase plays a role in viral replication and that this role extends beyond its function of activating cdc2 for initiation of the ICP22-dependent cascade for upregulation of gamma(2) gene expression.
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页码:4527 / 4532
页数:6
相关论文
共 20 条
[1]   The role of cdc2 in the expression of herpes simplex virus genes [J].
Advani, SJ ;
Weichselbaum, RR ;
Roizman, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (20) :10996-11001
[2]   The disappearance of cyclins A and B and the increase in activity of the G2/M-phase cellular kinase cdc2 in herpes simplex virus 1-infected cells require expression of the α22/US1.5 and UL13 viral genes [J].
Advani, SJ ;
Brandimarti, R ;
Weichselbaum, RR ;
Roizman, B .
JOURNAL OF VIROLOGY, 2000, 74 (01) :8-15
[3]   Herpes simplex virus 1 activates cdc2 to recruit topoisomerase IIα for post-DNA synthesis expression of late genes [J].
Advani, SJ ;
Weichselbaum, RR ;
Roizman, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (08) :4825-4830
[4]   Posttranslational processing of infected cell proteins 0 and 4 of herpes simplex virus 1 is sequential and reflects the subcellular compartment in which the proteins localize [J].
Advani, SJ ;
Hagglund, R ;
Weichselbaum, RR ;
Roizman, B .
JOURNAL OF VIROLOGY, 2001, 75 (17) :7904-7912
[5]   cdc2 cyclin-dependent kinase binds and phosphorylates herpes simplex virus 1 UL42 DNA synthesis processivity factor [J].
Advani, SJ ;
Weichselbaum, RR ;
Roizman, B .
JOURNAL OF VIROLOGY, 2001, 75 (21) :10326-10333
[6]   CELL-CYCLE REGULATION OF THE P34(CDC2) INHIBITORY KINASES [J].
ATHERTONFESSLER, S ;
LIU, F ;
GABRIELLI, B ;
LEE, MS ;
PENG, CY ;
PIWNICAWORMS, H .
MOLECULAR BIOLOGY OF THE CELL, 1994, 5 (09) :989-1001
[7]   THE HERPES-SIMPLEX VIRUS-1 U(L)15 GENE ENCODES 2 PROTEINS AND IS REQUIRED FOR CLEAVAGE OF GENOMIC VIRAL-DNA [J].
BAINES, JD ;
POON, APW ;
ROVNAK, J ;
ROIZMAN, B .
JOURNAL OF VIROLOGY, 1994, 68 (12) :8118-8124
[8]  
Berkelman T., 1998, 2 D ELECTROPHORESIS
[9]   ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS - PRINCIPLE, METHODOLOGY AND SOME APPLICATIONS [J].
BJELLQVIST, B ;
EK, K ;
RIGHETTI, PG ;
GIANAZZA, E ;
GORG, A ;
WESTERMEIER, R ;
POSTEL, W .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1982, 6 (04) :317-339
[10]   Absence of apparent phenotype in mice lacking Cdc25C protein phosphatase [J].
Chen, MS ;
Hurov, J ;
White, LS ;
Woodford-Thomas, T ;
Piwnica-WormS, H .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (12) :3853-3861