JOINT 3D ALIGNMENT-RECONSTRUCTION MULTI-SCALE APPROACH FOR CRYO ELECTRON TOMOGRAPHY

被引：1

作者：

Rojbani, Hmida ^{[1
,2
]}

Baudrier, Etienne ^{[1
]}

Naegel, Benoit ^{[1
]}

Mazo, Loic ^{[1
]}

Hamouda, Atef ^{[2
]}

机构：

[1] Univ Strasbourg, CNRS ICube, Illkirch Graffenstaden, France

[2] Univ Tunis ElManar, LIPAH, Tunis, Tunisia

来源：

2016 IEEE 13TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI) | 2016年

关键词：

Electron tomography; 3D reconstruction; Geometric transformation uncertainty; Conjugate Gradient; Multi-scale; 3-DIMENSIONAL RECONSTRUCTION; TILT SERIES; TEM;

D O I：

10.1109/ISBI.2016.7493460

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

3D volume reconstruction in cryo-electron tomography is possible by using Transmission Electron Microscope (TEM) images from different tilt angles. The misalignment of these images is one of the limits to the quality of the reconstructed object. There are many alignment techniques to deal with this problem. Their common feature is to correct the 2D geometric transformation in the projection images. Nevertheless, 3D geometric transformation can occur in the TEM acquisition including tilt angular uncertainty. In this paper, we proposed a new multi-scale approach based on a Conjugate Gradient optimization of a cost function between the 3D reconstructed and the projection images with the purpose to find all the 3D parameters of geometric transformation. Tests on synthetic and real data prove the accuracy of our geometric transformation estimation.

引用

页码：1109 / 1113

页数：5

共 23 条

[21] Inverse Problem Approach for the Alignment of Electron Tomographic Series [J].

Tran, V. -D. ;

Moreaud, M. ;

Thiebaut, E. ;

Denis, L. ;

Becker, J. M. .

OIL & GAS SCIENCE AND TECHNOLOGY-REVUE D IFP ENERGIES NOUVELLES, 2014, 69 (02) :279-291

[22] A new generation of the IMAGIC image processing system [J].

vanHeel, M ;

Harauz, G ;

Orlova, EV ;

Schmidt, R ;

Schatz, M .

JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (01) :17-24

[23] AUTOMATED DATA COLLECTION FOR ELECTRON MICROSCOPIC TOMOGRAPHY [J].

Zheng, Shawn Q. ;

Sedat, J. W. ;

Agard, D. A. .

METHODS IN ENZYMOLOGY, VOL 481: CRYO-EM, PART A - SAMPLE PREPARATION AND DATA COLLECTION, 2010, 481 :283-315

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