Human L1 retrotransposon encodes a conserved endonuclease required for retrotransposition

被引：892
作者：
Feng, QH
Moran, JV
Kazazian, HH
Boeke, JD
机构：
[1] JOHNS HOPKINS UNIV,DEPT MOL BIOL & GENET,SCH MED,BALTIMORE,MD 21205
[2] UNIV PENN,SCH MED,DEPT GENET,PHILADELPHIA,PA 19104
来源：
CELL | 1996年 / 87卷 / 05期
关键词：
D O I：
10.1016/S0092-8674(00)81997-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human L1 elements are highly abundant poly(A) (non-LTR) retrotransposons whose second open reading frame (ORF2) encodes a reverse transcriptase (RT). We have identified an endonuclease (EN) domain at the L1 ORF2 N-terminus that is highly conserved among poly(A) retrotransposons and resembles the apurinic/apyrimidinic (AP) endonucleases. Purified L1 EN protein (L1 ENp) makes 5'-PO4, 3'-OH nicks in supercoiled plasmids, shows no preference for AP sites, and preferentially cleaves sequences resembling L1 in vivo target sequences. Mutations in conserved amino acid residues of L1 EN abolish its nicking activity and eliminate L1 retrotransposition. We propose that L1 EN cleaves the target site for L1 insertion and primes reverse transcription.
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页码：905 / 916
页数：12
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