The knowledge of Anthomyzidae (Diptera: Acalyptratae) in the East Palaearctic area is extended by new taxonomic, biological and distributional information. Two new species of the genus Anthomyza Fallen, 1810, viz. A. aspina sp. nov. (Russia: Far East) and A. breviclavus sp. nov. (North Korea), and a species tentatively affi liated with the genus Zealantha Rohacek, 2007, viz. Z. fasciolata sp. nov. (Japan) are described. Although all are based only on females, they are distinctive species unmistakably recognized by external characters and structures of the female postabdomen. Relationships of all new species are discussed. Records of 24 species are presented; 3 species are recorded from the E. Palae-arctic, 1 species from continental Asia, 3 species from North Korea and a number of species from particular parts of E. Palaearctic Russia for the first time. An updated checklist of East Palaearctic Anthomyzidae is presented, now comprising 13 genera and 44 species. Taxonomic notes for Anthomyza clara Rohacek, 2006, Fungomyza cercata Rohacek, 2009 and some other species, and new biological information for Anthomyza trifurca Sueyoshi & Rohacek, 2003 are given. Longitudinal dark patterning of the wing found in A. brevicla-vus sp. nov., represents the fi fth lineage of Anthomyzidae in which this type of pattern has evolved independently. Species richness of Anthomyzidae in the E. Palaearctic is discussed with an estimate of about 60 species occurring in the area.