Influence of cucumariosides upon intracellular [Ca 2+]i and lysosomal activity of macrophages

被引:23
作者
Agafonova, IG [1 ]
Aminin, DL [1 ]
Avilov, SA [1 ]
Stonik, VA [1 ]
机构
[1] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far Eastern Branch, Vladivostok 690022, Russia
关键词
sea cucumber; triterpene glycosides; cucumariosides; Ca2+ signaling; cytotoxicity; immunomodulation; lysosomal activity; mechanism of action;
D O I
10.1021/jf034439x
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Biological effects of the triterpene glycosides, cucumariosides A(2)-2 and A(7)-1 from the edible sea cucumber Cucumaria japonica and their aglycones were investigated using embryos of the sea urchin Strongylocentrotus nudus and the BALB/C line mouse peritoneal macrophages. Cucumariosides were highly cytotoxic in a sea urchin embryo development test with EC50 values of 0.3 and 1.98 mug/mL, respectively. The aglycone was completely lacking in cytotoxicity. In subtoxic concentrations (0.001-0.1 mug/mL), cucumarioside A(2)-2 showed more then 2-fold stimulation of lysosomal activity and induced a rapid short-term increase in cytosolic Ca2+ Content in mouse macrophages. The maximal stimulatory effect was detected after 1-2 h of cultivation of cells with this glycoside. Cucumarioside A(7)-1 demonstrated more weak effects and even slightly inhibited lysosomal activity, while the aglycone was completely ineffective. At the toxic concentration (1 mug/mL), cucumarioside A(2)-2 induced the sharp irreversable increase of intracellular Ca2+ concentration. We suggested that cucumariosides, especially A(2)-2, may act as Ca2+ agonists due to their membranolytic properties.
引用
收藏
页码:6982 / 6986
页数:5
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