Detection of Mycoplasma spp. and Ureaplasma diversum in cown with reproductive disorders

In the study, 112 samples of vulvovaginal mucus of cows bearing reproductive disturbance were investigated for Mycoplasma and Ureaplasma. Specific media for the culture of mycoplasmas (SP-4) and ureaplasmas were used PCR with general primers, PCR specific for Mycoplasma bovis, and nested-PCR in a single tube for Ureaplasma diversum were performed to detect DNA of the sample. Mycoplasma spp. and U. diversum were isolated in 12.5 and 25.0%, respectively. With generic PCR, positive reaction was obtained in 63.4% of the samples transported in SP-4 and 69.6% in ureaplasma medium. M. bovis was detected in 9.8% of samples and nested-PCR in a single tube for U. diversum resulted in 35.0% of positive reaction. Results demonstrated increased sensitiviiy of PCR methodology compared with culture technique applied to the search of microorganisms of Mycoplasma and Ureasplasma genera.