Quantitative metaproteomics of medieval dental calculus reveals individual oral health status

被引：63

作者：

Jersie-Christensen, Rosa R. ^{[1
]}

Lanigan, Liam T. ^{[2
]}

Lyon, David ^{[3
]}

Mackie, Meaghan ^{[1
,2
]}

Belstrom, Daniel ^{[4
]}

Kelstrup, Christian D. ^{[1
]}

Fotakis, Anna K. ^{[2
]}

Willerslev, Eske ^{[5
,6
]}

Lynnerup, Niels ^{[7
]}

Jensen, Lars J. ^{[3
]}

Cappellini, Enrico ^{[2
]}

Olsen, Jesper V. ^{[1
]}

机构：

[1] Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn, Prote Program,Ctr Prot Res, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark

[2] Univ Copenhagen, Nat Hist Museum Denmark, Oster Voldgade 5-7, DK-1350 Copenhagen K, Denmark

[3] Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn, Dis Syst Biol Program,Ctr Prot Res, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark

[4] Univ Copenhagen, Fac Hlth Sci, Dept Odontol, Periodontol & Microbiol, Norre Alle 20, DK-2200 Copenhagen N, Denmark

[5] Univ Copenhagen, Nat Hist Museum Denmark, Ctr GeoGenet, Oster Voldgade 5-7, DK-1350 Copenhagen K, Denmark

[6] Univ Cambridge, Dept Zool, Downing St, Cambridge CB2 3EJ, England

[7] Univ Copenhagen, Fac Hlth Sci, Inst Forens Med, Lab Biol Anthropol, Frederik Vs Vej 11, DK-2100 Copenhagen O, Denmark

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

新加坡国家研究基金会;

关键词：

CANDIDATE PERIODONTAL PATHOGENS; INFECTIVE ENDOCARDITIS; BACTERIAL DIVERSITY; MICROBIOME; IDENTIFICATION; ASSOCIATION; PLAQUE; WEAR;

D O I：

10.1038/s41467-018-07148-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The composition of ancient oral microbiomes has recently become accessible owing to advanced biomolecular methods such as metagenomics and metaproteomics, but the utility of metaproteomics for such analyses is less explored. Here, we use quantitative metaproteomics to characterize the dental calculus associated with the remains of 21 humans retrieved during the archeological excavation of the medieval (ca. 1100-1450 CE) cemetery of Tjaerby, Denmark. We identify 3671 protein groups, covering 220 bacterial species and 81 genera across all medieval samples. The metaproteome profiles of bacterial and human proteins suggest two distinct groups of archeological remains corresponding to health-predisposed and oral disease-susceptible individuals, which is supported by comparison to the calculus metaproteomes of healthy living individuals. Notably, the groupings identified by metaproteomics are not apparent from the bioarchaeological analysis, illustrating that quantitative metaproteomics has the potential to provide additional levels of molecular information about the oral health status of individuals from archeological contexts.

引用

页数：12

共 63 条

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