Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis

被引:37
作者
Sun, Yinghua [1 ,2 ]
Sun, Yang [1 ]
Stephens, Douglas [1 ]
Xie, Hongtao [1 ]
Phipps, Jennifer [1 ]
Saroufeem, Ramez [3 ]
Southard, Jeffrey [4 ]
Elson, Daniel S. [5 ]
Marcu, Laura [1 ,2 ]
机构
[1] Univ Calif Davis, Dept Biomed Engn, Davis, CA 95616 USA
[2] Univ Calif Davis, NSF Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA
[3] Dept Med Pathol & Lab Med, Sacramento, CA 95817 USA
[4] UC Davis Med Ctr, Div Cardiovasc Med, Sacramento, CA 95817 USA
[5] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England
基金
美国国家卫生研究院;
关键词
OPTICAL SPECTROSCOPY; LESIONS;
D O I
10.1364/OE.19.003890
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands using dichroic filters, with each sub-band coupled into a different length of optical fiber for temporal separation. STWRFS allows for fast recording/ analysis (few microseconds) of time-resolved fluorescence emission in these sub-bands and rapid scanning. Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm. Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter. Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis. (C) 2011 Optical Society of America
引用
收藏
页码:3890 / 3901
页数:12
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