EBPR Using Crude Glycerol: Assessing Process Resiliency and Exploring Metabolic Anomalies

被引:19
作者
Coats, Erik R. [1 ]
Dobroth, Zachary T.
Brinkman, Cynthia K. [1 ]
机构
[1] Univ Idaho, Dept Civil Engn, Moscow, ID 83844 USA
关键词
enhanced biological phosphorus removal (EBPR); crude glycerol; polyphosphate accumulating organisms (PAOs); glycogen accumulating organisms (GAOs); volatile fatty acids (VFAs); polyphosphate; BIOLOGICAL PHOSPHORUS REMOVAL; GLYCOGEN-ACCUMULATING ORGANISMS; ACTIVATED-SLUDGE; WASTE-WATER; POSTANOXIC DENITRIFICATION; CARBON-SOURCES; ACETATE UPTAKE; STOICHIOMETRY; KINETICS; SCALE;
D O I
10.2175/106143014X14062131179113
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Enhanced biological phosphorus removal (EBPR) is predicated on exposing bacteria to cyclical anaerobic/aerobic environments while providing volatile fatty acids (VFAs). Combined, this environment enriches for phosphorus accumulating organisms (PAOs) and induces metabolisms to ensure excess phosphorus removal. Crude glycerol (CG), a byproduct of biodiesel manufacturing, is an alternate waste stream that could be substituted to achieve excess phosphorus removal; research into the use of CG yielded unexpected findings. While CG was an excellent substrate to accomplish and/or help achieve excess phosphorus removal, CG-fed bacteria did not consistently exhibit theoretical EBPR metabolisms. Specifically, anaerobic phosphorus release was not required for successful EBPR; however, carbon cycling patterns were consistent with theory. Analysis of results suggests that PAOs will first leverage carbon to generate energy anaerobically; only as needed will the bacteria utilize polyphosphate reserves anaerobically. Results also demonstrated that excess phosphorus removal can be achieved with a small fraction of PAOs.
引用
收藏
页码:68 / 79
页数:12
相关论文
共 41 条
[1]   NITRATE AND NITRITE REDUCTIONS WITH ANAEROBIC SLUDGE USING VARIOUS CARBON-SOURCES - GLUCOSE, GLYCEROL, ACETIC-ACID, LACTIC-ACID AND METHANOL [J].
AKUNNA, JC ;
BIZEAU, C ;
MOLETTA, R .
WATER RESEARCH, 1993, 27 (08) :1303-1312
[2]   The role of the microbial stringent response in excess intracellular accumulation of phosphorous in mixed consortia fed synthetic wastewater [J].
Al-Najjar, Muamar M. ;
Coats, Erik R. ;
Loge, Frank J. .
WATER RESEARCH, 2011, 45 (16) :5038-5046
[3]   Bacterial poly(hydroxyalkanoate) polymer production from the biodiesel co-product stream [J].
Ashby, RD ;
Solaiman, DKY ;
Foglia, TA .
JOURNAL OF POLYMERS AND THE ENVIRONMENT, 2004, 12 (03) :105-112
[4]   Polyhydroxyalkanoates, biopolyesters from renewable resources: Physiological and engineering aspects [J].
Braunegg, G ;
Lefebvre, G ;
Genser, KF .
JOURNAL OF BIOTECHNOLOGY, 1998, 65 (2-3) :127-161
[5]  
Clesceri L.S., 1998, STANDARD METHODS EXA, V20th, DOI DOI 10.2105/AJPH.56.3.387
[6]   A Comparative Environmental Life-Cycle Analysis for Removing Phosphorus from Wastewater: Biological versus Physical/Chemical Processes [J].
Coats, Erik R. ;
Watkins, David L. ;
Kranenburg, Dan .
WATER ENVIRONMENT RESEARCH, 2011, 83 (08) :750-760
[7]   Effect of Anaerobic HRT on Biological Phosphorus Removal and the Enrichment of Phosphorus Accumulating Organisms [J].
Coats, Erik R. ;
Watkins, David L. ;
Brinkman, Cynthia K. ;
Loge, Frank J. .
WATER ENVIRONMENT RESEARCH, 2011, 83 (05) :461-469
[8]   Post-anoxic denitrification driven by PHA and glycogen within enhanced biological phosphorus removal [J].
Coats, Erik R. ;
Mockos, Alexander ;
Loge, Frank J. .
BIORESOURCE TECHNOLOGY, 2011, 102 (02) :1019-1027
[9]   Glycogen-accumulating organisms in laboratory-scale and full-scale wastewater treatment processes [J].
Crocetti, GR ;
Banfield, JF ;
Keller, J ;
Bond, PL ;
Blackall, LL .
MICROBIOLOGY-SGM, 2002, 148 :3353-3364
[10]   DGGE with genomic DNA: Suitable for detection of numerically important organisms but not for identification of the most abundant organisms [J].
de Araujo, Juliana Calabria ;
Schneider, Rene Peter .
WATER RESEARCH, 2008, 42 (20) :5002-5010