Transcription factor DegU-mediated multi-pathway regulation on lichenysin biosynthesis in Bacillus licheniformis

被引:22
作者
Hu, Shiying [1 ]
He, Penghui [1 ]
Zhang, Yongjia [1 ]
Jiang, Meng [1 ]
Wang, Qin [1 ]
Yang, Shihui [1 ]
Chen, Shouwen [1 ]
机构
[1] Hubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Environm Microbial Technol Ctr Hubei Prov, Wuhan 430062, Peoples R China
关键词
Bacillus licheniformis; Lichenysin; DegU; Glucose uptake; BCAAs synthesis; Fatty acid synthesis; Acetoin synthesis; BACILLOMYCIN-D; FLUX; FRAMEWORK; PROMOTER; OPERON; COLI;
D O I
10.1016/j.ymben.2022.10.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lichenysin, producted by Bacillus licheniformis, is an important cyclic lipopeptide biosurfactant, which has po-tential applications in oil exploitation, drug development, biological control of agriculture and bioremediation. While studies are lacking on metabolism regulation of lichenysin biosynthesis, which limits metabolic engi-neering and large-scale production of lichenysin. In this study, the yield of lichenysin was improved obviously by 13.6 folds to 2.18 +/- 0.03 g/L in degU deletion strain (WX02odegU) compared with the wild-type strain (WX02) and completely inhibited in degU overexpressed strain (WX02/pHY-degU). We further proved that DegU, a transcription factor plays a significant role in multicellular behavior, is a key negative regulator of lichenysin synthesis lchA operon. But interestingly, lichenysin yield was still inhibited by overexpressing DegU in the promoter-substituted strain (WX02-PP43lch), in which promoter of lchA operon cannot be controlled by DegU. Thus, through 13C-metabolic flux analysis, we found that deletion of degU also enhanced glucose uptake, branched chain amino acid synthesis, and fatty acid synthesis, while decrease acetoin synthesis, which is beneficial for the supply of lichenysin precursors. Further experiments demonstrate that DegU regulates these pathways by binding to the promoter regions of related genes. Overall, we systematically investigated the multi-pathway regulation network mediated by DegU on lichenysin biosynthesis, which not only contributes to the further metabolic engineering for lichenysin high-production, but sheds light on studies of transcription factor regulation.
引用
收藏
页码:108 / 120
页数:13
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