Restoration of gap junctional intercellular communication by caffeic acid phenethyl ester (CAPE) in a ras-transformed rat liver epithelial cell line

被引：64
作者：
Na, HK
Wilson, MR
Kang, KS
Chang, CC
Grunberger, D
Trosko, JE [1 ]
机构：
[1] Michigan State Univ, Dept Pediat & Human Dev, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA
[2] Seoul Natl Univ, Coll Vet Med, Dept Publ Hlth, Suwon 441744, South Korea
[3] Columbia Univ Coll Phys & Surg, Inst Canc Res, New York, NY 10032 USA
来源：
CANCER LETTERS | 2000年 / 157卷 / 01期
关键词：
caffeic acid phenethyl ester; gap junctional intercellular communication; transformation by ras oncogene;
D O I：
10.1016/S0304-3835(00)00470-5
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Caffeic acid phenethyl ester (CAPE), an active ingredient of honeybee propolis, has been identified as having anti-inflammatory, anti-viral and anti-cancer properties. Since the deficiency of gap junctional intercellular communication (GJIC) has been shown to be a characteristic of most cancer cells, this study was designed to test the hypothesis that the anti-carcinogenic activity of CAFE might be related to its ability to restore GJIC in tumorigenic GJIC-deficient cells (WB-ras2 cells). The results showed that CAFE restored GJIC, phosphorylation of connexin 43 (Cx43) and its normal localization on the plasma membrane in WB-ras2 cells after 3 days at 5 mug/ml concentration. Additionally, CAFE inhibited growth in soft agar and decreased the protein level of p21(ras). The results are consistent with the hypothesis that the anti-cancer mechanism of CAFE may be mediated by its ability to restore GJIC. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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页码：31 / 38
页数：8
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